Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism

Dendritic cells (DC) are a subset of leukocytes whose major function is antigen presentation. We investigated the phenotype and function of enriched (95–98.5%) rat DC. We show that both spleen and thymus DC express the natural killer cell receptor protein 1 (NKR-P1) as a disulfide linked homodimer o...

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Autores principales: Josien, Régis, Heslan, Michèle, Soulillou, Jean-Paul, Cuturi, Maria-Cristina
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Brief Definitive Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2198993/
https://www.ncbi.nlm.nih.gov/pubmed/9236200
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author Josien, Régis
Heslan, Michèle
Soulillou, Jean-Paul
Cuturi, Maria-Cristina
author_facet Josien, Régis
Heslan, Michèle
Soulillou, Jean-Paul
Cuturi, Maria-Cristina
author_sort Josien, Régis
collection PubMed
description Dendritic cells (DC) are a subset of leukocytes whose major function is antigen presentation. We investigated the phenotype and function of enriched (95–98.5%) rat DC. We show that both spleen and thymus DC express the natural killer cell receptor protein 1 (NKR-P1) as a disulfide linked homodimer of 60 kD. Freshly isolated DC express a low level of NKR-P1, which is strongly upregulated after overnight culture. Spleen, but not thymus DC, were able to kill the NK-sensitive YAC-1 cell line in vitro, and since this killing was Ca(2+) dependent, a Fas ligand–Fas interaction was probably not involved. Besides their potent antigen-presenting function, DC can thus be cytotoxic for some tumor targets.
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spelling pubmed-21989932008-04-16 Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism Josien, Régis Heslan, Michèle Soulillou, Jean-Paul Cuturi, Maria-Cristina J Exp Med Brief Definitive Report Dendritic cells (DC) are a subset of leukocytes whose major function is antigen presentation. We investigated the phenotype and function of enriched (95–98.5%) rat DC. We show that both spleen and thymus DC express the natural killer cell receptor protein 1 (NKR-P1) as a disulfide linked homodimer of 60 kD. Freshly isolated DC express a low level of NKR-P1, which is strongly upregulated after overnight culture. Spleen, but not thymus DC, were able to kill the NK-sensitive YAC-1 cell line in vitro, and since this killing was Ca(2+) dependent, a Fas ligand–Fas interaction was probably not involved. Besides their potent antigen-presenting function, DC can thus be cytotoxic for some tumor targets. The Rockefeller University Press 1997-08-04 /pmc/articles/PMC2198993/ /pubmed/9236200 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Josien, Régis
Heslan, Michèle
Soulillou, Jean-Paul
Cuturi, Maria-Cristina
Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title_full Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title_fullStr Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title_full_unstemmed Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title_short Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca(2+)-dependent Mechanism
title_sort rat spleen dendritic cells express natural killer cell receptor protein 1 (nkr-p1) and have cytotoxic activity to select targets via a ca(2+)-dependent mechanism
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2198993/
https://www.ncbi.nlm.nih.gov/pubmed/9236200
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