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Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves

The embryonic thymus is colonized by the influx of hemopoietic progenitors in waves. To characterize the T cell progeny of the initial colonization waves, we used intravenous adoptive transfer of bone marrow progenitors into congenic embryos. The experiments were performed in birds because intraveno...

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Autores principales: Dunon, D., Courtois, D., Vainio, O., Six, A., Chen, C.H., Cooper, M.D., Dangy, J.P., Imhof, B.A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199063/
https://www.ncbi.nlm.nih.gov/pubmed/9314548
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author Dunon, D.
Courtois, D.
Vainio, O.
Six, A.
Chen, C.H.
Cooper, M.D.
Dangy, J.P.
Imhof, B.A.
author_facet Dunon, D.
Courtois, D.
Vainio, O.
Six, A.
Chen, C.H.
Cooper, M.D.
Dangy, J.P.
Imhof, B.A.
author_sort Dunon, D.
collection PubMed
description The embryonic thymus is colonized by the influx of hemopoietic progenitors in waves. To characterize the T cell progeny of the initial colonization waves, we used intravenous adoptive transfer of bone marrow progenitors into congenic embryos. The experiments were performed in birds because intravenous cell infusions can be performed more efficiently in avian than in mammalian embryos. Progenitor cells, which entered the vascularized thymus via interlobular venules in the capsular region and capillaries located at the corticomedullary junction, homed to the outer cortex to begin thymocyte differentiation. The kinetics of differentiation and emigration of the T cell progeny were analyzed for the first three waves of progenitors. Each progenitor wave gave rise to γ/δ T cells 3 d earlier than α/β T cells. Although the flow of T cell migration from the thymus was uninterrupted, distinct colonization and differentiation kinetics defined three successive waves of γ/δ and α/β T cells that depart sequentially the thymus en route to the periphery. Each wave of precursors rearranged all three TCR Vγ gene families, but displayed a variable repertoire. The data indicate a complex pattern of repertoire diversification by the progeny of founder thymocyte progenitors.
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spelling pubmed-21990632008-04-16 Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves Dunon, D. Courtois, D. Vainio, O. Six, A. Chen, C.H. Cooper, M.D. Dangy, J.P. Imhof, B.A. J Exp Med Article The embryonic thymus is colonized by the influx of hemopoietic progenitors in waves. To characterize the T cell progeny of the initial colonization waves, we used intravenous adoptive transfer of bone marrow progenitors into congenic embryos. The experiments were performed in birds because intravenous cell infusions can be performed more efficiently in avian than in mammalian embryos. Progenitor cells, which entered the vascularized thymus via interlobular venules in the capsular region and capillaries located at the corticomedullary junction, homed to the outer cortex to begin thymocyte differentiation. The kinetics of differentiation and emigration of the T cell progeny were analyzed for the first three waves of progenitors. Each progenitor wave gave rise to γ/δ T cells 3 d earlier than α/β T cells. Although the flow of T cell migration from the thymus was uninterrupted, distinct colonization and differentiation kinetics defined three successive waves of γ/δ and α/β T cells that depart sequentially the thymus en route to the periphery. Each wave of precursors rearranged all three TCR Vγ gene families, but displayed a variable repertoire. The data indicate a complex pattern of repertoire diversification by the progeny of founder thymocyte progenitors. The Rockefeller University Press 1997-10-06 /pmc/articles/PMC2199063/ /pubmed/9314548 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Dunon, D.
Courtois, D.
Vainio, O.
Six, A.
Chen, C.H.
Cooper, M.D.
Dangy, J.P.
Imhof, B.A.
Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title_full Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title_fullStr Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title_full_unstemmed Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title_short Ontogeny of the Immune System: γ/δ and α/β T Cells Migrate from Thymus to the Periphery in Alternating Waves
title_sort ontogeny of the immune system: γ/δ and α/β t cells migrate from thymus to the periphery in alternating waves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199063/
https://www.ncbi.nlm.nih.gov/pubmed/9314548
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