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Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family

TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane–bound prot...

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Autores principales: Degli-Esposti, Mariapia A., Smolak, Pamela J., Walczak, Henning, Waugh, Jennifer, Huang, Chang-Pin, DuBose, Robert F., Goodwin, Raymond G., Smith, Craig A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199077/
https://www.ncbi.nlm.nih.gov/pubmed/9314565
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author Degli-Esposti, Mariapia A.
Smolak, Pamela J.
Walczak, Henning
Waugh, Jennifer
Huang, Chang-Pin
DuBose, Robert F.
Goodwin, Raymond G.
Smith, Craig A.
author_facet Degli-Esposti, Mariapia A.
Smolak, Pamela J.
Walczak, Henning
Waugh, Jennifer
Huang, Chang-Pin
DuBose, Robert F.
Goodwin, Raymond G.
Smith, Craig A.
author_sort Degli-Esposti, Mariapia A.
collection PubMed
description TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane–bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol–linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis.
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spelling pubmed-21990772008-04-16 Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family Degli-Esposti, Mariapia A. Smolak, Pamela J. Walczak, Henning Waugh, Jennifer Huang, Chang-Pin DuBose, Robert F. Goodwin, Raymond G. Smith, Craig A. J Exp Med Brief Definitive Report TRAIL-R3, a new member of the TRAIL receptor family, has been cloned and characterized. TRAIL-R3 encodes a 299 amino acid protein with 58 and 54% overall identity to TRAIL-R1 and -R2, respectively. Transient expression and quantitative binding studies show TRAIL-R3 to be a plasma membrane–bound protein capable of high affinity interaction with the TRAIL ligand. The TRAIL-R3 gene maps to human chromosome 8p22-21, clustered with the genes encoding two other TRAIL receptors. In contrast to TRAIL-R1 and -R2, this receptor shows restricted expression, with transcripts detectable only in peripheral blood lymphocytes and spleen. The structure of TRAIL-R3 is unique when compared to the other TRAIL receptors in that it lacks a cytoplasmic domain and appears to be glycosyl-phosphatidylinositol–linked. Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis. The Rockefeller University Press 1997-10-06 /pmc/articles/PMC2199077/ /pubmed/9314565 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Degli-Esposti, Mariapia A.
Smolak, Pamela J.
Walczak, Henning
Waugh, Jennifer
Huang, Chang-Pin
DuBose, Robert F.
Goodwin, Raymond G.
Smith, Craig A.
Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title_full Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title_fullStr Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title_full_unstemmed Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title_short Cloning and Characterization of TRAIL-R3, a Novel Member of the Emerging TRAIL Receptor Family
title_sort cloning and characterization of trail-r3, a novel member of the emerging trail receptor family
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199077/
https://www.ncbi.nlm.nih.gov/pubmed/9314565
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