Cargando…
Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity
Heat shock protein (HSP) preparations derived from cancer cells and virus-infected cells have been shown previously to elicit cancer-specific or virus-specific immunity. The immunogenicity of HSP preparations has been attributed to peptides associated with the HSPs. The studies reported here demonst...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199095/ https://www.ncbi.nlm.nih.gov/pubmed/9334371 |
_version_ | 1782148133047762944 |
---|---|
author | Blachere, Nathalie E. Li, Zihai Chandawarkar, Rajiv Y. Suto, Ryuichiro Jaikaria, Navdeep S. Basu, Sreyashi Udono, Heiichiro Srivastava, Pramod K. |
author_facet | Blachere, Nathalie E. Li, Zihai Chandawarkar, Rajiv Y. Suto, Ryuichiro Jaikaria, Navdeep S. Basu, Sreyashi Udono, Heiichiro Srivastava, Pramod K. |
author_sort | Blachere, Nathalie E. |
collection | PubMed |
description | Heat shock protein (HSP) preparations derived from cancer cells and virus-infected cells have been shown previously to elicit cancer-specific or virus-specific immunity. The immunogenicity of HSP preparations has been attributed to peptides associated with the HSPs. The studies reported here demonstrate that immunogenic HSP–peptide complexes can also be reconstituted in vitro. The studies show that (a) complexes of hsp70 or gp96 HSP molecules with a variety of synthetic peptides can be generated in vitro; (b) the binding of HSPs with peptides is specific in that a number of other proteins tested do not bind synthetic peptides under the conditions in which gp96 molecules do; (c) HSP–peptide complexes reconstituted in vitro are immunologically active, as tested by their ability to elicit antitumor immunity and specific CD8(+) cytolytic T lymphocyte response; and (d) synthetic peptides reconstituted in vitro with gp96 are capable of being taken up and re-presented by macrophage in the same manner as gp96– peptides complexes generated in vivo. These observations demonstrate that HSPs are CD8(+) T cell response–eliciting adjuvants. |
format | Text |
id | pubmed-2199095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21990952008-04-16 Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity Blachere, Nathalie E. Li, Zihai Chandawarkar, Rajiv Y. Suto, Ryuichiro Jaikaria, Navdeep S. Basu, Sreyashi Udono, Heiichiro Srivastava, Pramod K. J Exp Med Article Heat shock protein (HSP) preparations derived from cancer cells and virus-infected cells have been shown previously to elicit cancer-specific or virus-specific immunity. The immunogenicity of HSP preparations has been attributed to peptides associated with the HSPs. The studies reported here demonstrate that immunogenic HSP–peptide complexes can also be reconstituted in vitro. The studies show that (a) complexes of hsp70 or gp96 HSP molecules with a variety of synthetic peptides can be generated in vitro; (b) the binding of HSPs with peptides is specific in that a number of other proteins tested do not bind synthetic peptides under the conditions in which gp96 molecules do; (c) HSP–peptide complexes reconstituted in vitro are immunologically active, as tested by their ability to elicit antitumor immunity and specific CD8(+) cytolytic T lymphocyte response; and (d) synthetic peptides reconstituted in vitro with gp96 are capable of being taken up and re-presented by macrophage in the same manner as gp96– peptides complexes generated in vivo. These observations demonstrate that HSPs are CD8(+) T cell response–eliciting adjuvants. The Rockefeller University Press 1997-10-20 /pmc/articles/PMC2199095/ /pubmed/9334371 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Blachere, Nathalie E. Li, Zihai Chandawarkar, Rajiv Y. Suto, Ryuichiro Jaikaria, Navdeep S. Basu, Sreyashi Udono, Heiichiro Srivastava, Pramod K. Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title | Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title_full | Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title_fullStr | Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title_full_unstemmed | Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title_short | Heat Shock Protein–Peptide Complexes, Reconstituted In Vitro, Elicit Peptide-specific Cytotoxic T Lymphocyte Response and Tumor Immunity |
title_sort | heat shock protein–peptide complexes, reconstituted in vitro, elicit peptide-specific cytotoxic t lymphocyte response and tumor immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199095/ https://www.ncbi.nlm.nih.gov/pubmed/9334371 |
work_keys_str_mv | AT blacherenathaliee heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT lizihai heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT chandawarkarrajivy heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT sutoryuichiro heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT jaikarianavdeeps heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT basusreyashi heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT udonoheiichiro heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity AT srivastavapramodk heatshockproteinpeptidecomplexesreconstitutedinvitroelicitpeptidespecificcytotoxictlymphocyteresponseandtumorimmunity |