Cargando…
Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice
The role of antibodies (Abs) in the development of chronic colitis in T cell receptor (TCR)-α(−/−) mice was explored by creating double mutant mice (TCR-α(−/−) × immunoglobulin (Ig)μ(−/−)), which lack B cells. TCR-α(−/−) × Igμ(−/−) mice spontaneously developed colitis at an earlier age, and the coli...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199135/ https://www.ncbi.nlm.nih.gov/pubmed/9362534 |
_version_ | 1782148142460829696 |
---|---|
author | Mizoguchi, Atsushi Mizoguchi, Emiko Smith, R. Neal Preffer, Frederic I. Bhan, Atul K. |
author_facet | Mizoguchi, Atsushi Mizoguchi, Emiko Smith, R. Neal Preffer, Frederic I. Bhan, Atul K. |
author_sort | Mizoguchi, Atsushi |
collection | PubMed |
description | The role of antibodies (Abs) in the development of chronic colitis in T cell receptor (TCR)-α(−/−) mice was explored by creating double mutant mice (TCR-α(−/−) × immunoglobulin (Ig)μ(−/−)), which lack B cells. TCR-α(−/−) × Igμ(−/−) mice spontaneously developed colitis at an earlier age, and the colitis was more severe than in TCR-α(−/−) mice. Colitis was induced in recombination-activating gene-1 (RAG-1(−/−)) mice by the transfer of mesenteric lymph node (MLN) cells from TCR-α(−/−) × Igμ(−/−) mice. When purified B cells from TCR-α(−/−) mice were mixed with MLN cells before cell transfer, colitis did not develop in RAG-1(−/−) mice. Administration of the purified Ig from TCR-α(−/−) mice and a mixture of monoclonal autoAbs reactive with colonic epithelial cells led to attenuation of colitis in TCR-α(−/−) × Igμ(−/−) mice. Apoptotic cells were increased in the colon, MLN, and spleen of TCR-α(−/−) × Igμ(−/−) mice as compared to Igμ(−/−) mice and TCR-α(−/−) mice. Administration of the purified Ig from TCR-α(−/−) mice into TCR-α(−/−) × Igμ(−/−) mice led to decrease in the number of apoptotic cells. These findings suggest that although B cells are not required for the initiation of colitis, B cells and Igs (autoAbs) can suppress colitis, presumably by affecting the clearance of apoptotic cells. |
format | Text |
id | pubmed-2199135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21991352008-04-16 Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice Mizoguchi, Atsushi Mizoguchi, Emiko Smith, R. Neal Preffer, Frederic I. Bhan, Atul K. J Exp Med Article The role of antibodies (Abs) in the development of chronic colitis in T cell receptor (TCR)-α(−/−) mice was explored by creating double mutant mice (TCR-α(−/−) × immunoglobulin (Ig)μ(−/−)), which lack B cells. TCR-α(−/−) × Igμ(−/−) mice spontaneously developed colitis at an earlier age, and the colitis was more severe than in TCR-α(−/−) mice. Colitis was induced in recombination-activating gene-1 (RAG-1(−/−)) mice by the transfer of mesenteric lymph node (MLN) cells from TCR-α(−/−) × Igμ(−/−) mice. When purified B cells from TCR-α(−/−) mice were mixed with MLN cells before cell transfer, colitis did not develop in RAG-1(−/−) mice. Administration of the purified Ig from TCR-α(−/−) mice and a mixture of monoclonal autoAbs reactive with colonic epithelial cells led to attenuation of colitis in TCR-α(−/−) × Igμ(−/−) mice. Apoptotic cells were increased in the colon, MLN, and spleen of TCR-α(−/−) × Igμ(−/−) mice as compared to Igμ(−/−) mice and TCR-α(−/−) mice. Administration of the purified Ig from TCR-α(−/−) mice into TCR-α(−/−) × Igμ(−/−) mice led to decrease in the number of apoptotic cells. These findings suggest that although B cells are not required for the initiation of colitis, B cells and Igs (autoAbs) can suppress colitis, presumably by affecting the clearance of apoptotic cells. The Rockefeller University Press 1997-11-17 /pmc/articles/PMC2199135/ /pubmed/9362534 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Mizoguchi, Atsushi Mizoguchi, Emiko Smith, R. Neal Preffer, Frederic I. Bhan, Atul K. Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title | Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title_full | Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title_fullStr | Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title_full_unstemmed | Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title_short | Suppressive Role of B Cells in Chronic Colitis of T Cell Receptor α Mutant Mice |
title_sort | suppressive role of b cells in chronic colitis of t cell receptor α mutant mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199135/ https://www.ncbi.nlm.nih.gov/pubmed/9362534 |
work_keys_str_mv | AT mizoguchiatsushi suppressiveroleofbcellsinchroniccolitisoftcellreceptoramutantmice AT mizoguchiemiko suppressiveroleofbcellsinchroniccolitisoftcellreceptoramutantmice AT smithrneal suppressiveroleofbcellsinchroniccolitisoftcellreceptoramutantmice AT prefferfrederici suppressiveroleofbcellsinchroniccolitisoftcellreceptoramutantmice AT bhanatulk suppressiveroleofbcellsinchroniccolitisoftcellreceptoramutantmice |