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Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation

We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, w...

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Detalles Bibliográficos
Autores principales: Neumann, Harald, Schmidt, Hannes, Wilharm, Elke, Behrens, Lüder, Wekerle, Hartmut
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199162/
https://www.ncbi.nlm.nih.gov/pubmed/9396771
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author Neumann, Harald
Schmidt, Hannes
Wilharm, Elke
Behrens, Lüder
Wekerle, Hartmut
author_facet Neumann, Harald
Schmidt, Hannes
Wilharm, Elke
Behrens, Lüder
Wekerle, Hartmut
author_sort Neumann, Harald
collection PubMed
description We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN-γ protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN-γ and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN-γ receptor. Locally produced IFN-γ acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN-γ–dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-γ. Our findings indicate a role of IFN-γ in autocrine regulation of sensory neurons.
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spelling pubmed-21991622008-04-16 Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation Neumann, Harald Schmidt, Hannes Wilharm, Elke Behrens, Lüder Wekerle, Hartmut J Exp Med Article We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN-γ protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN-γ and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN-γ receptor. Locally produced IFN-γ acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN-γ–dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-γ. Our findings indicate a role of IFN-γ in autocrine regulation of sensory neurons. The Rockefeller University Press 1997-12-15 /pmc/articles/PMC2199162/ /pubmed/9396771 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Neumann, Harald
Schmidt, Hannes
Wilharm, Elke
Behrens, Lüder
Wekerle, Hartmut
Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title_full Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title_fullStr Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title_full_unstemmed Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title_short Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
title_sort interferon γ gene expression in sensory neurons: evidence for autocrine gene regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199162/
https://www.ncbi.nlm.nih.gov/pubmed/9396771
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