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Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, w...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199162/ https://www.ncbi.nlm.nih.gov/pubmed/9396771 |
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author | Neumann, Harald Schmidt, Hannes Wilharm, Elke Behrens, Lüder Wekerle, Hartmut |
author_facet | Neumann, Harald Schmidt, Hannes Wilharm, Elke Behrens, Lüder Wekerle, Hartmut |
author_sort | Neumann, Harald |
collection | PubMed |
description | We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN-γ protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN-γ and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN-γ receptor. Locally produced IFN-γ acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN-γ–dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-γ. Our findings indicate a role of IFN-γ in autocrine regulation of sensory neurons. |
format | Text |
id | pubmed-2199162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21991622008-04-16 Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation Neumann, Harald Schmidt, Hannes Wilharm, Elke Behrens, Lüder Wekerle, Hartmut J Exp Med Article We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN-γ protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN-γ and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN-γ receptor. Locally produced IFN-γ acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN-γ–dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-γ. Our findings indicate a role of IFN-γ in autocrine regulation of sensory neurons. The Rockefeller University Press 1997-12-15 /pmc/articles/PMC2199162/ /pubmed/9396771 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Neumann, Harald Schmidt, Hannes Wilharm, Elke Behrens, Lüder Wekerle, Hartmut Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title | Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title_full | Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title_fullStr | Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title_full_unstemmed | Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title_short | Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation |
title_sort | interferon γ gene expression in sensory neurons: evidence for autocrine gene regulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199162/ https://www.ncbi.nlm.nih.gov/pubmed/9396771 |
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