An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles

The release of biogenic amines from large dense core vesicles (LDCVs) depends on localization of the vesicular monoamine transporter VMAT2 to LDCVs. We now find that a cluster of acidic residues including two serines phosphorylated by casein kinase 2 is required for the localization of VMAT2 to LDCV...

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Autores principales: Waites, Clarissa L., Mehta, Anand, Tan, Philip K., Thomas, Gary, Edwards, Robert H., Krantz, David E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199206/
https://www.ncbi.nlm.nih.gov/pubmed/11257117
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author Waites, Clarissa L.
Mehta, Anand
Tan, Philip K.
Thomas, Gary
Edwards, Robert H.
Krantz, David E.
author_facet Waites, Clarissa L.
Mehta, Anand
Tan, Philip K.
Thomas, Gary
Edwards, Robert H.
Krantz, David E.
author_sort Waites, Clarissa L.
collection PubMed
description The release of biogenic amines from large dense core vesicles (LDCVs) depends on localization of the vesicular monoamine transporter VMAT2 to LDCVs. We now find that a cluster of acidic residues including two serines phosphorylated by casein kinase 2 is required for the localization of VMAT2 to LDCVs. Deletion of the acidic cluster promotes the removal of VMAT2 from LDCVs during their maturation. The motif thus acts as a signal for retention on LDCVs. In addition, replacement of the serines by glutamate to mimic phosphorylation promotes the removal of VMAT2 from LDCVs, whereas replacement by alanine to prevent phosphorylation decreases removal. Phosphorylation of the acidic cluster thus appears to reduce the localization of VMAT2 to LDCVs by inactivating a retention mechanism.
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spelling pubmed-21992062008-05-01 An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles Waites, Clarissa L. Mehta, Anand Tan, Philip K. Thomas, Gary Edwards, Robert H. Krantz, David E. J Cell Biol Original Article The release of biogenic amines from large dense core vesicles (LDCVs) depends on localization of the vesicular monoamine transporter VMAT2 to LDCVs. We now find that a cluster of acidic residues including two serines phosphorylated by casein kinase 2 is required for the localization of VMAT2 to LDCVs. Deletion of the acidic cluster promotes the removal of VMAT2 from LDCVs during their maturation. The motif thus acts as a signal for retention on LDCVs. In addition, replacement of the serines by glutamate to mimic phosphorylation promotes the removal of VMAT2 from LDCVs, whereas replacement by alanine to prevent phosphorylation decreases removal. Phosphorylation of the acidic cluster thus appears to reduce the localization of VMAT2 to LDCVs by inactivating a retention mechanism. The Rockefeller University Press 2001-03-19 /pmc/articles/PMC2199206/ /pubmed/11257117 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Waites, Clarissa L.
Mehta, Anand
Tan, Philip K.
Thomas, Gary
Edwards, Robert H.
Krantz, David E.
An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title_full An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title_fullStr An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title_full_unstemmed An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title_short An Acidic Motif Retains Vesicular Monoamine Transporter 2 on Large Dense Core Vesicles
title_sort acidic motif retains vesicular monoamine transporter 2 on large dense core vesicles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199206/
https://www.ncbi.nlm.nih.gov/pubmed/11257117
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