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Macrophage podosomes assemble at the leading lamella by growth and fragmentation

Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin–ECFP and L-fimbrin–EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo po...

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Detalles Bibliográficos
Autores principales: Evans, James G., Correia, Ivan, Krasavina, Olga, Watson, Nicki, Matsudaira, Paul
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199349/
https://www.ncbi.nlm.nih.gov/pubmed/12756237
http://dx.doi.org/10.1083/jcb.200212037
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author Evans, James G.
Correia, Ivan
Krasavina, Olga
Watson, Nicki
Matsudaira, Paul
author_facet Evans, James G.
Correia, Ivan
Krasavina, Olga
Watson, Nicki
Matsudaira, Paul
author_sort Evans, James G.
collection PubMed
description Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin–ECFP and L-fimbrin–EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion.
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spelling pubmed-21993492008-05-01 Macrophage podosomes assemble at the leading lamella by growth and fragmentation Evans, James G. Correia, Ivan Krasavina, Olga Watson, Nicki Matsudaira, Paul J Cell Biol Report Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with β-actin–ECFP and L-fimbrin–EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosome cluster precursor also appears to be an adhesion structure; it contains actin, fimbrin, integrin, and is in close apposition to the substratum. Microtubule inhibitors paclitaxel and demecolcine inhibit the turnover and polarized formation of podosomes, but not the turnover rate of actin in these structures. Because daughter podosomes and podosome cluster precursors are preferentially located at the leading edge, they may play a critical role in continually generating new sites of cell adhesion. The Rockefeller University Press 2003-05-26 /pmc/articles/PMC2199349/ /pubmed/12756237 http://dx.doi.org/10.1083/jcb.200212037 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Evans, James G.
Correia, Ivan
Krasavina, Olga
Watson, Nicki
Matsudaira, Paul
Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title_full Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title_fullStr Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title_full_unstemmed Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title_short Macrophage podosomes assemble at the leading lamella by growth and fragmentation
title_sort macrophage podosomes assemble at the leading lamella by growth and fragmentation
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199349/
https://www.ncbi.nlm.nih.gov/pubmed/12756237
http://dx.doi.org/10.1083/jcb.200212037
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