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A Cell-Free Assay Allows Reconstitution of Vps33p-Dependent Transport to the Yeast Vacuole/Lysosome

We report a cell-free system that measures transport-coupled maturation of carboxypeptidase Y (CPY). Yeast spheroplasts are lysed by extrusion through polycarbonate filters. After differential centrifugation, a 125,000-g pellet is enriched for radiolabeled proCPY and is used as “donor” membranes. A...

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Detalles Bibliográficos
Autores principales:	Vida, Thomas, Gerhardt, Brenda
Formato:	Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 1999
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199724/ https://www.ncbi.nlm.nih.gov/pubmed/10402462

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