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Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
We have cloned and characterized the troponin C gene, pat-10 of the nematode Caenorhabditis elegans. At the amino acid level nematode troponin C is most similar to troponin C of Drosophila (45% identity) and cardiac troponin C of vertebrates. Expression studies demonstrate that this troponin is expr...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199735/ https://www.ncbi.nlm.nih.gov/pubmed/10402470 |
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author | Terami, Hiromi Williams, Benjamin D. Kitamura, Shin-ichi Sakube, Yasuji Matsumoto, Shinji Doi, Shima Obinata, Takashi Kagawa, Hiroaki |
author_facet | Terami, Hiromi Williams, Benjamin D. Kitamura, Shin-ichi Sakube, Yasuji Matsumoto, Shinji Doi, Shima Obinata, Takashi Kagawa, Hiroaki |
author_sort | Terami, Hiromi |
collection | PubMed |
description | We have cloned and characterized the troponin C gene, pat-10 of the nematode Caenorhabditis elegans. At the amino acid level nematode troponin C is most similar to troponin C of Drosophila (45% identity) and cardiac troponin C of vertebrates. Expression studies demonstrate that this troponin is expressed in body wall muscle throughout the life of the animal. Later, vulval muscles and anal muscles also express this troponin C isoform. The structural gene for this troponin is pat-10 and mutations in this gene lead to animals that arrest as twofold paralyzed embryos late in development. We have sequenced two of the mutations in pat-10 and both had identical two mutations in the gene; one changes D64 to N and the other changes W153 to a termination site. The missense alteration affects a calcium-binding site and eliminates calcium binding, whereas the second mutation eliminates binding to troponin I. These combined biochemical and in vivo studies of mutant animals demonstrate that this troponin is essential for proper muscle function during development. |
format | Text |
id | pubmed-2199735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21997352008-05-01 Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans Terami, Hiromi Williams, Benjamin D. Kitamura, Shin-ichi Sakube, Yasuji Matsumoto, Shinji Doi, Shima Obinata, Takashi Kagawa, Hiroaki J Cell Biol Original Article We have cloned and characterized the troponin C gene, pat-10 of the nematode Caenorhabditis elegans. At the amino acid level nematode troponin C is most similar to troponin C of Drosophila (45% identity) and cardiac troponin C of vertebrates. Expression studies demonstrate that this troponin is expressed in body wall muscle throughout the life of the animal. Later, vulval muscles and anal muscles also express this troponin C isoform. The structural gene for this troponin is pat-10 and mutations in this gene lead to animals that arrest as twofold paralyzed embryos late in development. We have sequenced two of the mutations in pat-10 and both had identical two mutations in the gene; one changes D64 to N and the other changes W153 to a termination site. The missense alteration affects a calcium-binding site and eliminates calcium binding, whereas the second mutation eliminates binding to troponin I. These combined biochemical and in vivo studies of mutant animals demonstrate that this troponin is essential for proper muscle function during development. The Rockefeller University Press 1999-07-12 /pmc/articles/PMC2199735/ /pubmed/10402470 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Terami, Hiromi Williams, Benjamin D. Kitamura, Shin-ichi Sakube, Yasuji Matsumoto, Shinji Doi, Shima Obinata, Takashi Kagawa, Hiroaki Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans |
title | Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
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title_full | Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
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title_fullStr | Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
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title_full_unstemmed | Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
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title_short | Genomic Organization, Expression, and Analysis of the Troponin C Gene pat-10 of Caenorhabditis elegans
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title_sort | genomic organization, expression, and analysis of the troponin c gene pat-10 of caenorhabditis elegans |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2199735/ https://www.ncbi.nlm.nih.gov/pubmed/10402470 |
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