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Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions
The NUM1 gene is involved in the control of nuclear migration in Saccharomyces cerevisiae. The content of NUM1 mRNA fluctuates during the cell cycle, reaching a maximum at S/G2 phase, and the translation product Num1p associates with the cortex of mother cells mainly during S, G2, and mitosis, as se...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1995
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200000/ https://www.ncbi.nlm.nih.gov/pubmed/7490278 |
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collection | PubMed |
description | The NUM1 gene is involved in the control of nuclear migration in Saccharomyces cerevisiae. The content of NUM1 mRNA fluctuates during the cell cycle, reaching a maximum at S/G2 phase, and the translation product Num1p associates with the cortex of mother cells mainly during S, G2, and mitosis, as seen by indirect immunofluorescence. The nuclear spindle in NUM1-deficient large-budded cells often fails to align along the mother/bud axis, while abnormally elongated astral microtubules emanate from both spindle pole bodies. A num1 null mutation confers temperature sensitivity to the cold-sensitive alpha-tubulin mutant tub1- 1, and shows synthetic lethality with the beta-tubulin mutant alleles tub2-402, tub2-403, tub2-404, and tub2-405. Deletion mapping has defined three functionally important Num1p regions: a potential EF hand Ca2+ binding site, a cluster of potential phosphorylation sites and a pleckstrin homology domain. The latter domain appears to be involved in targeting Num1p to the mother cell cortex. Our data suggest that the periodically expressed NUM1 gene product controls nuclear migration by affecting astral microtubule functions. |
format | Text |
id | pubmed-2200000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22000002008-05-01 Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions J Cell Biol Articles The NUM1 gene is involved in the control of nuclear migration in Saccharomyces cerevisiae. The content of NUM1 mRNA fluctuates during the cell cycle, reaching a maximum at S/G2 phase, and the translation product Num1p associates with the cortex of mother cells mainly during S, G2, and mitosis, as seen by indirect immunofluorescence. The nuclear spindle in NUM1-deficient large-budded cells often fails to align along the mother/bud axis, while abnormally elongated astral microtubules emanate from both spindle pole bodies. A num1 null mutation confers temperature sensitivity to the cold-sensitive alpha-tubulin mutant tub1- 1, and shows synthetic lethality with the beta-tubulin mutant alleles tub2-402, tub2-403, tub2-404, and tub2-405. Deletion mapping has defined three functionally important Num1p regions: a potential EF hand Ca2+ binding site, a cluster of potential phosphorylation sites and a pleckstrin homology domain. The latter domain appears to be involved in targeting Num1p to the mother cell cortex. Our data suggest that the periodically expressed NUM1 gene product controls nuclear migration by affecting astral microtubule functions. The Rockefeller University Press 1995-11-02 /pmc/articles/PMC2200000/ /pubmed/7490278 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title | Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title_full | Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title_fullStr | Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title_full_unstemmed | Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title_short | Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions |
title_sort | yeast num1p associates with the mother cell cortex during s/g2 phase and affects microtubular functions |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200000/ https://www.ncbi.nlm.nih.gov/pubmed/7490278 |