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Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina
The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neura...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1993
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200109/ https://www.ncbi.nlm.nih.gov/pubmed/8468350 |
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collection | PubMed |
description | The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. Strikingly, culture of RPE results in rapid redistribution of N-CAM to the basolateral surface. This is not due to an isoform shift, since the N-CAM expressed by cultured cells (140 kD) is the same as that expressed by RPE in vivo. Rather, the reversed polarity of N-CAM appears to result from the disruption of the contact between the RPE and the photoreceptors of the neural retina. We suggest that N-CAM in RPE and photoreceptors participate in these interactions. |
format | Text |
id | pubmed-2200109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22001092008-05-01 Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina J Cell Biol Articles The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. Strikingly, culture of RPE results in rapid redistribution of N-CAM to the basolateral surface. This is not due to an isoform shift, since the N-CAM expressed by cultured cells (140 kD) is the same as that expressed by RPE in vivo. Rather, the reversed polarity of N-CAM appears to result from the disruption of the contact between the RPE and the photoreceptors of the neural retina. We suggest that N-CAM in RPE and photoreceptors participate in these interactions. The Rockefeller University Press 1993-04-02 /pmc/articles/PMC2200109/ /pubmed/8468350 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title | Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title_full | Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title_fullStr | Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title_full_unstemmed | Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title_short | Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina |
title_sort | apical polarization of n-cam in retinal pigment epithelium is dependent on contact with the neural retina |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200109/ https://www.ncbi.nlm.nih.gov/pubmed/8468350 |