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Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection

Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). H...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200125/
https://www.ncbi.nlm.nih.gov/pubmed/8227132
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collection PubMed
description Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC- specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110- kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not.
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spelling pubmed-22001252008-05-01 Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection J Cell Biol Articles Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC- specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110- kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not. The Rockefeller University Press 1993-11-01 /pmc/articles/PMC2200125/ /pubmed/8227132 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title_full Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title_fullStr Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title_full_unstemmed Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title_short Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
title_sort roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200125/
https://www.ncbi.nlm.nih.gov/pubmed/8227132