Cargando…
Reconstitution of nuclear protein transport with semi-intact yeast cells
We have developed an in vitro nuclear protein import reaction from semi- intact yeast cells. The reaction uses cells that have been permeabilized by freeze-thaw after spheroplast formation. Electron microscopic analysis and antibody-binding experiments show that the nuclear envelope remains intact b...
| Formato: | Texto |
|---|---|
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1993
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200159/ https://www.ncbi.nlm.nih.gov/pubmed/8227140 |