IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA

When Chlamydomonas reinhardi is supplied with (methyl-(3)H)-thymidine, radioactivity is incorporated specifically into chloroplast DNA Chromatographic analysis of the products of enzymatic hydrolysis of the DNA reveals that only thymidine monophosphate has been labeled. Use of thymidine-6-(3)H yield...

Descripción completa

Detalles Bibliográficos
Autores principales: Swinton, David C., Hanawalt, Philip C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200293/
https://www.ncbi.nlm.nih.gov/pubmed/5044760
_version_ 1782148289116766208
author Swinton, David C.
Hanawalt, Philip C.
author_facet Swinton, David C.
Hanawalt, Philip C.
author_sort Swinton, David C.
collection PubMed
description When Chlamydomonas reinhardi is supplied with (methyl-(3)H)-thymidine, radioactivity is incorporated specifically into chloroplast DNA Chromatographic analysis of the products of enzymatic hydrolysis of the DNA reveals that only thymidine monophosphate has been labeled. Use of thymidine-6-(3)H yields an identical result. If thymidine-(3)H monophosphate is supplied, a small amount of radioactivity is incorporated into both nuclear and chloroplast DNA in proportion to the abundance of these DNA components. These observations are consistent with earlier suggestions that algae lack cytoplasmic thymidine kinase, but that the enzyme is present within their chloroplasts.
format Text
id pubmed-2200293
institution National Center for Biotechnology Information
language English
publishDate 1972
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22002932008-05-01 IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA Swinton, David C. Hanawalt, Philip C. J Cell Biol Article When Chlamydomonas reinhardi is supplied with (methyl-(3)H)-thymidine, radioactivity is incorporated specifically into chloroplast DNA Chromatographic analysis of the products of enzymatic hydrolysis of the DNA reveals that only thymidine monophosphate has been labeled. Use of thymidine-6-(3)H yields an identical result. If thymidine-(3)H monophosphate is supplied, a small amount of radioactivity is incorporated into both nuclear and chloroplast DNA in proportion to the abundance of these DNA components. These observations are consistent with earlier suggestions that algae lack cytoplasmic thymidine kinase, but that the enzyme is present within their chloroplasts. The Rockefeller University Press 1972-09-01 /pmc/articles/PMC2200293/ /pubmed/5044760 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Swinton, David C.
Hanawalt, Philip C.
IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title_full IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title_fullStr IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title_full_unstemmed IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title_short IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNA
title_sort in vivo specific labeling of chlamydomonas chloroplast dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2200293/
https://www.ncbi.nlm.nih.gov/pubmed/5044760
work_keys_str_mv AT swintondavidc invivospecificlabelingofchlamydomonaschloroplastdna
AT hanawaltphilipc invivospecificlabelingofchlamydomonaschloroplastdna

Ejemplares similares