Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments

Isolated human red blood cell membrane fragments (RBCMF) were found to take up Ca(++) in the presence of ATP.(1) This ATP-dependent Ca(++) uptake by RBCMF appears to be the manifestation of an active Ca(++) transport mechanism in the red cell membrane reported previously (Schatzmann, 1966; Lee and S...

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Autores principales: Cha, Young N., Shin, Bak C., Lee, Kwang S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203076/
https://www.ncbi.nlm.nih.gov/pubmed/5543418
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author Cha, Young N.
Shin, Bak C.
Lee, Kwang S.
author_facet Cha, Young N.
Shin, Bak C.
Lee, Kwang S.
author_sort Cha, Young N.
collection PubMed
description Isolated human red blood cell membrane fragments (RBCMF) were found to take up Ca(++) in the presence of ATP.(1) This ATP-dependent Ca(++) uptake by RBCMF appears to be the manifestation of an active Ca(++) transport mechanism in the red cell membrane reported previously (Schatzmann, 1966; Lee and Shin, 1969). The influences of altering experimental conditions on Ca(++)-stimulated Mg(++) ATPase (Ca(++) ATPase) and Ca(++) uptake of RBCMF were studied. It was found that pretreatment of RBCMF at 50°C abolished both Ca(++) ATPase and Ca(++) uptake. Pretreatment of RBCMF with phospholipases A and C decreased both Ca(++) ATPase and Ca(++) uptake, whereas pretreatment with phospholipase D did not significantly alter either Ca(++) ATPase or Ca(++) uptake. Both Ca(++) ATPase and Ca(++) uptake had ATP specificity, similar optimum pH's, and optimum incubation temperatures. From these results, it was concluded that Ca(++) uptake is intimately linked to Ca(++) ATPase.
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spelling pubmed-22030762008-04-23 Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments Cha, Young N. Shin, Bak C. Lee, Kwang S. J Gen Physiol Article Isolated human red blood cell membrane fragments (RBCMF) were found to take up Ca(++) in the presence of ATP.(1) This ATP-dependent Ca(++) uptake by RBCMF appears to be the manifestation of an active Ca(++) transport mechanism in the red cell membrane reported previously (Schatzmann, 1966; Lee and Shin, 1969). The influences of altering experimental conditions on Ca(++)-stimulated Mg(++) ATPase (Ca(++) ATPase) and Ca(++) uptake of RBCMF were studied. It was found that pretreatment of RBCMF at 50°C abolished both Ca(++) ATPase and Ca(++) uptake. Pretreatment of RBCMF with phospholipases A and C decreased both Ca(++) ATPase and Ca(++) uptake, whereas pretreatment with phospholipase D did not significantly alter either Ca(++) ATPase or Ca(++) uptake. Both Ca(++) ATPase and Ca(++) uptake had ATP specificity, similar optimum pH's, and optimum incubation temperatures. From these results, it was concluded that Ca(++) uptake is intimately linked to Ca(++) ATPase. The Rockefeller University Press 1971-02-01 /pmc/articles/PMC2203076/ /pubmed/5543418 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cha, Young N.
Shin, Bak C.
Lee, Kwang S.
Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title_full Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title_fullStr Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title_full_unstemmed Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title_short Active Uptake of Ca(++) and Ca(++)-Activated Mg(++) ATPase in Red Cell Membrane Fragments
title_sort active uptake of ca(++) and ca(++)-activated mg(++) atpase in red cell membrane fragments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203076/
https://www.ncbi.nlm.nih.gov/pubmed/5543418
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