Cargando…

Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia

Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synth...

Descripción completa

Detalles Bibliográficos
Autor principal: Wilson, David L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203096/
https://www.ncbi.nlm.nih.gov/pubmed/4099509
_version_ 1782148360506966016
author Wilson, David L.
author_facet Wilson, David L.
author_sort Wilson, David L.
collection PubMed
description Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.
format Text
id pubmed-2203096
institution National Center for Biotechnology Information
language English
publishDate 1971
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22030962008-04-23 Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia Wilson, David L. J Gen Physiol Article Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells. The Rockefeller University Press 1971-01-01 /pmc/articles/PMC2203096/ /pubmed/4099509 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wilson, David L.
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title_full Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title_fullStr Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title_full_unstemmed Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title_short Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
title_sort molecular weight distribution of proteins synthesized in single, identified neurons of aplysia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203096/
https://www.ncbi.nlm.nih.gov/pubmed/4099509
work_keys_str_mv AT wilsondavidl molecularweightdistributionofproteinssynthesizedinsingleidentifiedneuronsofaplysia