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The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells
2,4,6-Trinitro-3-methyl-phenol (trinitrocresol, H(+)TNC(-)) was found to inhibit anion and stimulate cation movements across the membranes of both high potassium (HK) and low potassium (LK) sheep red blood cells. The concentration of TNC(-) required to inhibit SO(4) (-) and Cl(-) efflux (10(-5)-10(-...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203116/ https://www.ncbi.nlm.nih.gov/pubmed/5553103 |
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author | Gunn, Robert B. Tosteson, Daniel C. |
author_facet | Gunn, Robert B. Tosteson, Daniel C. |
author_sort | Gunn, Robert B. |
collection | PubMed |
description | 2,4,6-Trinitro-3-methyl-phenol (trinitrocresol, H(+)TNC(-)) was found to inhibit anion and stimulate cation movements across the membranes of both high potassium (HK) and low potassium (LK) sheep red blood cells. The concentration of TNC(-) required to inhibit SO(4) (-) and Cl(-) efflux (10(-5)-10(-3) M) was less than that required to increase Na(+) and K(+) leakage (10(-3)-10(-2) M). Both the inhibition of anion and stimulation of cation permeation were reversed if TNC(-) was washed from the red cells. The cation leak caused by TNC(-) was much greater at 0° and 37°C than at room temperature (23°C). In sheep red cells, TNC(-) was found to be about 20 times more effective than salicylate and about 40 times more effective than thiocyanate in increasing cation leak. TNC(-) also inhibited the ouabain-sensitive potassium influx. |
format | Text |
id | pubmed-2203116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22031162008-04-23 The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells Gunn, Robert B. Tosteson, Daniel C. J Gen Physiol Article 2,4,6-Trinitro-3-methyl-phenol (trinitrocresol, H(+)TNC(-)) was found to inhibit anion and stimulate cation movements across the membranes of both high potassium (HK) and low potassium (LK) sheep red blood cells. The concentration of TNC(-) required to inhibit SO(4) (-) and Cl(-) efflux (10(-5)-10(-3) M) was less than that required to increase Na(+) and K(+) leakage (10(-3)-10(-2) M). Both the inhibition of anion and stimulation of cation permeation were reversed if TNC(-) was washed from the red cells. The cation leak caused by TNC(-) was much greater at 0° and 37°C than at room temperature (23°C). In sheep red cells, TNC(-) was found to be about 20 times more effective than salicylate and about 40 times more effective than thiocyanate in increasing cation leak. TNC(-) also inhibited the ouabain-sensitive potassium influx. The Rockefeller University Press 1971-05-01 /pmc/articles/PMC2203116/ /pubmed/5553103 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Gunn, Robert B. Tosteson, Daniel C. The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title | The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title_full | The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title_fullStr | The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title_full_unstemmed | The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title_short | The Effect of 2,4,6-Trinitro-m-Cresol on Cation and Anion Transport in Sheep Red Blood Cells |
title_sort | effect of 2,4,6-trinitro-m-cresol on cation and anion transport in sheep red blood cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203116/ https://www.ncbi.nlm.nih.gov/pubmed/5553103 |
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