Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues

The epithelium of the circumvallate papillae of rat tongues was stripped off by treatment with 0.005% elastase in a state when many taste buds were present. The taste buds were isolated from the stripped epithelium by further treatment with 0.005% elastase and 0.08% trypsin. A protein which was thou...

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Detalles Bibliográficos
Autor principal: Uehara, Seiji
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203451/
https://www.ncbi.nlm.nih.gov/pubmed/4570121
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author Uehara, Seiji
author_facet Uehara, Seiji
author_sort Uehara, Seiji
collection PubMed
description The epithelium of the circumvallate papillae of rat tongues was stripped off by treatment with 0.005% elastase in a state when many taste buds were present. The taste buds were isolated from the stripped epithelium by further treatment with 0.005% elastase and 0.08% trypsin. A protein which was thought to be characteristic of taste buds was found from semimicro disc polyacrylamide gel electrophoretic studies of the stripped epithelia with and without taste buds. This result was supported by micro disc polyacrylamide gel electrophoretic studies of isolated taste buds.
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spelling pubmed-22034512008-04-23 Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues Uehara, Seiji J Gen Physiol Article The epithelium of the circumvallate papillae of rat tongues was stripped off by treatment with 0.005% elastase in a state when many taste buds were present. The taste buds were isolated from the stripped epithelium by further treatment with 0.005% elastase and 0.08% trypsin. A protein which was thought to be characteristic of taste buds was found from semimicro disc polyacrylamide gel electrophoretic studies of the stripped epithelia with and without taste buds. This result was supported by micro disc polyacrylamide gel electrophoretic studies of isolated taste buds. The Rockefeller University Press 1973-03-01 /pmc/articles/PMC2203451/ /pubmed/4570121 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Uehara, Seiji
Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title_full Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title_fullStr Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title_full_unstemmed Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title_short Disc Electrophoresis of Extracts from the Taste Buds Located in Circumvallate Papillae of Rat Tongues
title_sort disc electrophoresis of extracts from the taste buds located in circumvallate papillae of rat tongues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203451/
https://www.ncbi.nlm.nih.gov/pubmed/4570121
work_keys_str_mv AT ueharaseiji discelectrophoresisofextractsfromthetastebudslocatedincircumvallatepapillaeofrattongues

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