Calcium-Dependent Myosin from Insect Flight Muscles

Calcium regulation of the insect actomyosin ATPase is associated with the thin filaments as in vertebrate muscles, and also with the myosin molecule as in mollusks. This dual regulation is demonstrated using combinations of locust thin filaments with rabbit myosin and locust myosin with rabbit actin...

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Detalles Bibliográficos
Autores principales: Lehman, William, Bullard, Belinda, Hammond, Kathleen
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203566/
https://www.ncbi.nlm.nih.gov/pubmed/4274833
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author Lehman, William
Bullard, Belinda
Hammond, Kathleen
author_facet Lehman, William
Bullard, Belinda
Hammond, Kathleen
author_sort Lehman, William
collection PubMed
description Calcium regulation of the insect actomyosin ATPase is associated with the thin filaments as in vertebrate muscles, and also with the myosin molecule as in mollusks. This dual regulation is demonstrated using combinations of locust thin filaments with rabbit myosin and locust myosin with rabbit actin; in each case the ATPase of the hybrid actomyosin is calcium dependent. The two regulatory systems are synergistic, the calcium dependency of the locust actomyosin ATPase being at least 10 times that of the hybrid actomyosins described above. Likewise Lethocerus myosin also contains regulatory proteins. The ATPase activity of Lethocerus myosin is labile and is stabilized by the presence of rabbit actin. Tropomyosin activates the ATPase of insect actomyosin and the activation occurs irrespective of whether the myosin is calcium dependent or rendered independent of calcium.
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spelling pubmed-22035662008-04-23 Calcium-Dependent Myosin from Insect Flight Muscles Lehman, William Bullard, Belinda Hammond, Kathleen J Gen Physiol Article Calcium regulation of the insect actomyosin ATPase is associated with the thin filaments as in vertebrate muscles, and also with the myosin molecule as in mollusks. This dual regulation is demonstrated using combinations of locust thin filaments with rabbit myosin and locust myosin with rabbit actin; in each case the ATPase of the hybrid actomyosin is calcium dependent. The two regulatory systems are synergistic, the calcium dependency of the locust actomyosin ATPase being at least 10 times that of the hybrid actomyosins described above. Likewise Lethocerus myosin also contains regulatory proteins. The ATPase activity of Lethocerus myosin is labile and is stabilized by the presence of rabbit actin. Tropomyosin activates the ATPase of insect actomyosin and the activation occurs irrespective of whether the myosin is calcium dependent or rendered independent of calcium. The Rockefeller University Press 1974-05-01 /pmc/articles/PMC2203566/ /pubmed/4274833 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Lehman, William
Bullard, Belinda
Hammond, Kathleen
Calcium-Dependent Myosin from Insect Flight Muscles
title Calcium-Dependent Myosin from Insect Flight Muscles
title_full Calcium-Dependent Myosin from Insect Flight Muscles
title_fullStr Calcium-Dependent Myosin from Insect Flight Muscles
title_full_unstemmed Calcium-Dependent Myosin from Insect Flight Muscles
title_short Calcium-Dependent Myosin from Insect Flight Muscles
title_sort calcium-dependent myosin from insect flight muscles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2203566/
https://www.ncbi.nlm.nih.gov/pubmed/4274833
work_keys_str_mv AT lehmanwilliam calciumdependentmyosinfrominsectflightmuscles
AT bullardbelinda calciumdependentmyosinfrominsectflightmuscles
AT hammondkathleen calciumdependentmyosinfrominsectflightmuscles

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