Optimal design and validation of antiviral siRNA for targeting HIV-1

We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regi...

Descripción completa

Detalles Bibliográficos
Autores principales: Naito, Yuki, Nohtomi, Kyoko, Onogi, Toshinari, Uenishi, Rie, Ui-Tei, Kumiko, Saigo, Kaoru, Takebe, Yutaka
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2204037/
https://www.ncbi.nlm.nih.gov/pubmed/17996047
http://dx.doi.org/10.1186/1742-4690-4-80
Descripción
Sumario:We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains.

Ejemplares similares