Optimal design and validation of antiviral siRNA for targeting HIV-1

We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regi...

Descripción completa

Detalles Bibliográficos
Autores principales: Naito, Yuki, Nohtomi, Kyoko, Onogi, Toshinari, Uenishi, Rie, Ui-Tei, Kumiko, Saigo, Kaoru, Takebe, Yutaka
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2204037/
https://www.ncbi.nlm.nih.gov/pubmed/17996047
http://dx.doi.org/10.1186/1742-4690-4-80
_version_ 1782148419980099584
author Naito, Yuki
Nohtomi, Kyoko
Onogi, Toshinari
Uenishi, Rie
Ui-Tei, Kumiko
Saigo, Kaoru
Takebe, Yutaka
author_facet Naito, Yuki
Nohtomi, Kyoko
Onogi, Toshinari
Uenishi, Rie
Ui-Tei, Kumiko
Saigo, Kaoru
Takebe, Yutaka
author_sort Naito, Yuki
collection PubMed
description We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains.
format Text
id pubmed-2204037
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-22040372008-01-17 Optimal design and validation of antiviral siRNA for targeting HIV-1 Naito, Yuki Nohtomi, Kyoko Onogi, Toshinari Uenishi, Rie Ui-Tei, Kumiko Saigo, Kaoru Takebe, Yutaka Retrovirology Short Report We propose rational designing of antiviral short-interfering RNA (siRNA) targeting highly divergent HIV-1. In this study, conserved regions within HIV-1 genomes were identified through an exhaustive computational analysis, and the functionality of siRNAs targeting the highest possible conserved regions was validated. We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains. BioMed Central 2007-11-08 /pmc/articles/PMC2204037/ /pubmed/17996047 http://dx.doi.org/10.1186/1742-4690-4-80 Text en Copyright © 2007 Naito et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Naito, Yuki
Nohtomi, Kyoko
Onogi, Toshinari
Uenishi, Rie
Ui-Tei, Kumiko
Saigo, Kaoru
Takebe, Yutaka
Optimal design and validation of antiviral siRNA for targeting HIV-1
title Optimal design and validation of antiviral siRNA for targeting HIV-1
title_full Optimal design and validation of antiviral siRNA for targeting HIV-1
title_fullStr Optimal design and validation of antiviral siRNA for targeting HIV-1
title_full_unstemmed Optimal design and validation of antiviral siRNA for targeting HIV-1
title_short Optimal design and validation of antiviral siRNA for targeting HIV-1
title_sort optimal design and validation of antiviral sirna for targeting hiv-1
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2204037/
https://www.ncbi.nlm.nih.gov/pubmed/17996047
http://dx.doi.org/10.1186/1742-4690-4-80
work_keys_str_mv AT naitoyuki optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT nohtomikyoko optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT onogitoshinari optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT uenishirie optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT uiteikumiko optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT saigokaoru optimaldesignandvalidationofantiviralsirnafortargetinghiv1
AT takebeyutaka optimaldesignandvalidationofantiviralsirnafortargetinghiv1

Ejemplares similares