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Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in s...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206013/ https://www.ncbi.nlm.nih.gov/pubmed/17988380 http://dx.doi.org/10.1186/1746-6148-3-29 |
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author | Niku, Mikael Pessa-Morikawa, Tiina Taponen, Juhani Iivanainen, Antti |
author_facet | Niku, Mikael Pessa-Morikawa, Tiina Taponen, Juhani Iivanainen, Antti |
author_sort | Niku, Mikael |
collection | PubMed |
description | BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in situ hybridization. RESULTS: Bull-derived CD34(+ )cells were detected in the liver of the female sibling (freemartin) at 60 days gestation. The level of bull-derived CD34(+ )cells was lower in the freemartin than in its male siblings. Bull (Y(+)) and cow hematopoietic cells often occurred in separate clusters. Around clusters of Y(+)CD34(+ )cells, Y(+)CD34(- )cells were typically observed. The thymi were also strongly chimeric at 60 days of gestation. CONCLUSION: The fetal freemartin liver contains clusters of bull-derived hematopoietic progenitors, suggesting clonal expansion and differentiation. Even the roots of the hematopoietic system in cattle twins are thus strongly chimeric from the early stages of fetal development. However, the hematopoietic seeding of fetal liver apparently started already before the onset of functional vascular anastomosis. |
format | Text |
id | pubmed-2206013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22060132008-01-18 Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle Niku, Mikael Pessa-Morikawa, Tiina Taponen, Juhani Iivanainen, Antti BMC Vet Res Research Article BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in situ hybridization. RESULTS: Bull-derived CD34(+ )cells were detected in the liver of the female sibling (freemartin) at 60 days gestation. The level of bull-derived CD34(+ )cells was lower in the freemartin than in its male siblings. Bull (Y(+)) and cow hematopoietic cells often occurred in separate clusters. Around clusters of Y(+)CD34(+ )cells, Y(+)CD34(- )cells were typically observed. The thymi were also strongly chimeric at 60 days of gestation. CONCLUSION: The fetal freemartin liver contains clusters of bull-derived hematopoietic progenitors, suggesting clonal expansion and differentiation. Even the roots of the hematopoietic system in cattle twins are thus strongly chimeric from the early stages of fetal development. However, the hematopoietic seeding of fetal liver apparently started already before the onset of functional vascular anastomosis. BioMed Central 2007-11-07 /pmc/articles/PMC2206013/ /pubmed/17988380 http://dx.doi.org/10.1186/1746-6148-3-29 Text en Copyright © 2007 Niku et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Niku, Mikael Pessa-Morikawa, Tiina Taponen, Juhani Iivanainen, Antti Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title | Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title_full | Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title_fullStr | Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title_full_unstemmed | Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title_short | Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
title_sort | direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206013/ https://www.ncbi.nlm.nih.gov/pubmed/17988380 http://dx.doi.org/10.1186/1746-6148-3-29 |
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