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Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle

BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in s...

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Detalles Bibliográficos
Autores principales: Niku, Mikael, Pessa-Morikawa, Tiina, Taponen, Juhani, Iivanainen, Antti
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206013/
https://www.ncbi.nlm.nih.gov/pubmed/17988380
http://dx.doi.org/10.1186/1746-6148-3-29
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author Niku, Mikael
Pessa-Morikawa, Tiina
Taponen, Juhani
Iivanainen, Antti
author_facet Niku, Mikael
Pessa-Morikawa, Tiina
Taponen, Juhani
Iivanainen, Antti
author_sort Niku, Mikael
collection PubMed
description BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in situ hybridization. RESULTS: Bull-derived CD34(+ )cells were detected in the liver of the female sibling (freemartin) at 60 days gestation. The level of bull-derived CD34(+ )cells was lower in the freemartin than in its male siblings. Bull (Y(+)) and cow hematopoietic cells often occurred in separate clusters. Around clusters of Y(+)CD34(+ )cells, Y(+)CD34(- )cells were typically observed. The thymi were also strongly chimeric at 60 days of gestation. CONCLUSION: The fetal freemartin liver contains clusters of bull-derived hematopoietic progenitors, suggesting clonal expansion and differentiation. Even the roots of the hematopoietic system in cattle twins are thus strongly chimeric from the early stages of fetal development. However, the hematopoietic seeding of fetal liver apparently started already before the onset of functional vascular anastomosis.
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spelling pubmed-22060132008-01-18 Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle Niku, Mikael Pessa-Morikawa, Tiina Taponen, Juhani Iivanainen, Antti BMC Vet Res Research Article BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in situ hybridization. RESULTS: Bull-derived CD34(+ )cells were detected in the liver of the female sibling (freemartin) at 60 days gestation. The level of bull-derived CD34(+ )cells was lower in the freemartin than in its male siblings. Bull (Y(+)) and cow hematopoietic cells often occurred in separate clusters. Around clusters of Y(+)CD34(+ )cells, Y(+)CD34(- )cells were typically observed. The thymi were also strongly chimeric at 60 days of gestation. CONCLUSION: The fetal freemartin liver contains clusters of bull-derived hematopoietic progenitors, suggesting clonal expansion and differentiation. Even the roots of the hematopoietic system in cattle twins are thus strongly chimeric from the early stages of fetal development. However, the hematopoietic seeding of fetal liver apparently started already before the onset of functional vascular anastomosis. BioMed Central 2007-11-07 /pmc/articles/PMC2206013/ /pubmed/17988380 http://dx.doi.org/10.1186/1746-6148-3-29 Text en Copyright © 2007 Niku et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Niku, Mikael
Pessa-Morikawa, Tiina
Taponen, Juhani
Iivanainen, Antti
Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title_full Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title_fullStr Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title_full_unstemmed Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title_short Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
title_sort direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206013/
https://www.ncbi.nlm.nih.gov/pubmed/17988380
http://dx.doi.org/10.1186/1746-6148-3-29
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