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Expression and function of junctional adhesion molecule-C in human and experimental arthritis
Junctional adhesion molecule-C (JAM-C) is an adhesion molecule involved in transendothelial migration of leukocytes. In this study, we examined JAM-C expression in the synovium and investigated the role of this molecule in two experimental mouse models of arthritis. JAM-C expression was investigated...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206366/ https://www.ncbi.nlm.nih.gov/pubmed/17612407 http://dx.doi.org/10.1186/ar2223 |
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author | Palmer, Gaby Busso, Nathalie Aurrand-Lions, Michel Talabot-Ayer, Dominique Chobaz-Péclat, Véronique Zimmerli, Claudia Hammel, Philippe Imhof, Beat A Gabay, Cem |
author_facet | Palmer, Gaby Busso, Nathalie Aurrand-Lions, Michel Talabot-Ayer, Dominique Chobaz-Péclat, Véronique Zimmerli, Claudia Hammel, Philippe Imhof, Beat A Gabay, Cem |
author_sort | Palmer, Gaby |
collection | PubMed |
description | Junctional adhesion molecule-C (JAM-C) is an adhesion molecule involved in transendothelial migration of leukocytes. In this study, we examined JAM-C expression in the synovium and investigated the role of this molecule in two experimental mouse models of arthritis. JAM-C expression was investigated by reverse transcriptase-polymerase chain reaction and immunohistochemistry. The effects of a monoclonal anti-JAM-C antibody were assessed in antigen-induced arthritis (AIA) and K/BxN serum transfer-induced arthritis. JAM-C was expressed by synovial fibroblasts in the lining layer and associated with vessels in the sublining layer in human and mouse arthritic synovial tissue. In human tissue, JAM-C expression was increased in rheumatoid arthritis (RA) as compared to osteoarthritis synovial samples (12.7 ± 1.3 arbitrary units in RA versus 3.3 ± 1.1 in OA; p < 0.05). Treatment of mice with a monoclonal anti-JAM-C antibody decreased the severity of AIA. Neutrophil infiltration into inflamed joints was selectively reduced as compared to T-lymphocyte and macrophage infiltration (0.8 ± 0.3 arbitrary units in anti-JAM-C-treated versus 2.3 ± 0.6 in isotype-matched control antibody-treated mice; p < 0.05). Circulating levels of the acute-phase protein serum amyloid A as well as antigen-specific and concanavalin A-induced spleen T-cell responses were significantly decreased in anti-JAM-C antibody-treated mice. In the serum transfer-induced arthritis model, treatment with the anti-JAM-C antibody delayed the onset of arthritis. JAM-C is highly expressed by synovial fibroblasts in RA. Treatment of mice with an anti-JAM-C antibody significantly reduced the severity of AIA and delayed the onset of serum transfer-induced arthritis, suggesting a role for JAM-C in the pathogenesis of arthritis. |
format | Text |
id | pubmed-2206366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22063662008-01-19 Expression and function of junctional adhesion molecule-C in human and experimental arthritis Palmer, Gaby Busso, Nathalie Aurrand-Lions, Michel Talabot-Ayer, Dominique Chobaz-Péclat, Véronique Zimmerli, Claudia Hammel, Philippe Imhof, Beat A Gabay, Cem Arthritis Res Ther Research Article Junctional adhesion molecule-C (JAM-C) is an adhesion molecule involved in transendothelial migration of leukocytes. In this study, we examined JAM-C expression in the synovium and investigated the role of this molecule in two experimental mouse models of arthritis. JAM-C expression was investigated by reverse transcriptase-polymerase chain reaction and immunohistochemistry. The effects of a monoclonal anti-JAM-C antibody were assessed in antigen-induced arthritis (AIA) and K/BxN serum transfer-induced arthritis. JAM-C was expressed by synovial fibroblasts in the lining layer and associated with vessels in the sublining layer in human and mouse arthritic synovial tissue. In human tissue, JAM-C expression was increased in rheumatoid arthritis (RA) as compared to osteoarthritis synovial samples (12.7 ± 1.3 arbitrary units in RA versus 3.3 ± 1.1 in OA; p < 0.05). Treatment of mice with a monoclonal anti-JAM-C antibody decreased the severity of AIA. Neutrophil infiltration into inflamed joints was selectively reduced as compared to T-lymphocyte and macrophage infiltration (0.8 ± 0.3 arbitrary units in anti-JAM-C-treated versus 2.3 ± 0.6 in isotype-matched control antibody-treated mice; p < 0.05). Circulating levels of the acute-phase protein serum amyloid A as well as antigen-specific and concanavalin A-induced spleen T-cell responses were significantly decreased in anti-JAM-C antibody-treated mice. In the serum transfer-induced arthritis model, treatment with the anti-JAM-C antibody delayed the onset of arthritis. JAM-C is highly expressed by synovial fibroblasts in RA. Treatment of mice with an anti-JAM-C antibody significantly reduced the severity of AIA and delayed the onset of serum transfer-induced arthritis, suggesting a role for JAM-C in the pathogenesis of arthritis. BioMed Central 2007 2007-07-05 /pmc/articles/PMC2206366/ /pubmed/17612407 http://dx.doi.org/10.1186/ar2223 Text en Copyright © 2007 Palmer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Palmer, Gaby Busso, Nathalie Aurrand-Lions, Michel Talabot-Ayer, Dominique Chobaz-Péclat, Véronique Zimmerli, Claudia Hammel, Philippe Imhof, Beat A Gabay, Cem Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title | Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title_full | Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title_fullStr | Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title_full_unstemmed | Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title_short | Expression and function of junctional adhesion molecule-C in human and experimental arthritis |
title_sort | expression and function of junctional adhesion molecule-c in human and experimental arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206366/ https://www.ncbi.nlm.nih.gov/pubmed/17612407 http://dx.doi.org/10.1186/ar2223 |
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