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Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus
Systemic lupus erythematosus (SLE) is associated with premature and accelerated atherosclerosis. Circulating progenitor cells (CPCs) are circulating bone-marrow derived cells that play an important role in the repair of vascular damage that underlies the development of atherosclerosis. The objective...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206388/ https://www.ncbi.nlm.nih.gov/pubmed/17764548 http://dx.doi.org/10.1186/ar2283 |
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author | Moonen, Jan Renier AJ de Leeuw, Karina van Seijen, Xavier J Gallego Y Kallenberg, Cees GM van Luyn, Marja JA Bijl, Marc Harmsen, Martin C |
author_facet | Moonen, Jan Renier AJ de Leeuw, Karina van Seijen, Xavier J Gallego Y Kallenberg, Cees GM van Luyn, Marja JA Bijl, Marc Harmsen, Martin C |
author_sort | Moonen, Jan Renier AJ |
collection | PubMed |
description | Systemic lupus erythematosus (SLE) is associated with premature and accelerated atherosclerosis. Circulating progenitor cells (CPCs) are circulating bone-marrow derived cells that play an important role in the repair of vascular damage that underlies the development of atherosclerosis. The objective of this study was to determine the number and functionality of CPCs in patients with SLE. The study included 44 female SLE patients in an inactive stage of disease and 35 age-matched female controls. CPC numbers in the circulation were determined by FACS with monoclonals against CD14, CD34 and CD133. Peripheral blood-derived mononuclear cell (PBMNC) fractions were cultured in angiogenic medium. The endothelial-like phenotype was confirmed and the colony forming unit (CFU) capacity, migratory capacity and the potential to form clusters on Matrigel were determined. Expression of apoptosis inhibiting caspase 8L was analyzed in PBMNCs and CPCs by gene transcript and protein expression assays. The number of CD34–CD133 double-positive cells (P < 0.001) as well as the CFU capacity (P = 0.048) was reduced in SLE patients. Migratory activity on tumor necrosis factor-α tended to be reduced in patient CPCs (P = 0.08). Migration on vascular endothelial growth factor showed no significant differences, nor were differences observed in the potential to form clusters on Matrigel. The expression of caspase 8L was reduced at the transcriptional level (P = 0.049) and strongly increased at the protein level after culture (P = 0.003). We conclude that CPC numbers are reduced in SLE patients and functionality is partly impaired. We suggest these findings reflect increased susceptibility to apoptosis of CPCs from SLE patients. |
format | Text |
id | pubmed-2206388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22063882008-01-19 Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus Moonen, Jan Renier AJ de Leeuw, Karina van Seijen, Xavier J Gallego Y Kallenberg, Cees GM van Luyn, Marja JA Bijl, Marc Harmsen, Martin C Arthritis Res Ther Research Article Systemic lupus erythematosus (SLE) is associated with premature and accelerated atherosclerosis. Circulating progenitor cells (CPCs) are circulating bone-marrow derived cells that play an important role in the repair of vascular damage that underlies the development of atherosclerosis. The objective of this study was to determine the number and functionality of CPCs in patients with SLE. The study included 44 female SLE patients in an inactive stage of disease and 35 age-matched female controls. CPC numbers in the circulation were determined by FACS with monoclonals against CD14, CD34 and CD133. Peripheral blood-derived mononuclear cell (PBMNC) fractions were cultured in angiogenic medium. The endothelial-like phenotype was confirmed and the colony forming unit (CFU) capacity, migratory capacity and the potential to form clusters on Matrigel were determined. Expression of apoptosis inhibiting caspase 8L was analyzed in PBMNCs and CPCs by gene transcript and protein expression assays. The number of CD34–CD133 double-positive cells (P < 0.001) as well as the CFU capacity (P = 0.048) was reduced in SLE patients. Migratory activity on tumor necrosis factor-α tended to be reduced in patient CPCs (P = 0.08). Migration on vascular endothelial growth factor showed no significant differences, nor were differences observed in the potential to form clusters on Matrigel. The expression of caspase 8L was reduced at the transcriptional level (P = 0.049) and strongly increased at the protein level after culture (P = 0.003). We conclude that CPC numbers are reduced in SLE patients and functionality is partly impaired. We suggest these findings reflect increased susceptibility to apoptosis of CPCs from SLE patients. BioMed Central 2007 2007-08-31 /pmc/articles/PMC2206388/ /pubmed/17764548 http://dx.doi.org/10.1186/ar2283 Text en Copyright © 2007 Moonen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Moonen, Jan Renier AJ de Leeuw, Karina van Seijen, Xavier J Gallego Y Kallenberg, Cees GM van Luyn, Marja JA Bijl, Marc Harmsen, Martin C Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title | Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title_full | Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title_fullStr | Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title_full_unstemmed | Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title_short | Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
title_sort | reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2206388/ https://www.ncbi.nlm.nih.gov/pubmed/17764548 http://dx.doi.org/10.1186/ar2283 |
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