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Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo

BACKGROUND: In bovine maturing oocytes and cleavage stage embryos, gene expression is mostly controlled at the post-transcriptional level, through degradation and deadenylation/polyadenylation. We have investigated how post transcriptional control of maternal transcripts was affected during in vitro...

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Autores principales: Thélie, Aurore, Papillier, Pascal, Pennetier, Sophie, Perreau, Christine, Traverso, Juan Martin, Uzbekova, Svetlana, Mermillod, Pascal, Joly, Catherine, Humblot, Patrice, Dalbiès-Tran, Rozenn
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211488/
https://www.ncbi.nlm.nih.gov/pubmed/17988387
http://dx.doi.org/10.1186/1471-213X-7-125
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author Thélie, Aurore
Papillier, Pascal
Pennetier, Sophie
Perreau, Christine
Traverso, Juan Martin
Uzbekova, Svetlana
Mermillod, Pascal
Joly, Catherine
Humblot, Patrice
Dalbiès-Tran, Rozenn
author_facet Thélie, Aurore
Papillier, Pascal
Pennetier, Sophie
Perreau, Christine
Traverso, Juan Martin
Uzbekova, Svetlana
Mermillod, Pascal
Joly, Catherine
Humblot, Patrice
Dalbiès-Tran, Rozenn
author_sort Thélie, Aurore
collection PubMed
description BACKGROUND: In bovine maturing oocytes and cleavage stage embryos, gene expression is mostly controlled at the post-transcriptional level, through degradation and deadenylation/polyadenylation. We have investigated how post transcriptional control of maternal transcripts was affected during in vitro and in vivo maturation, as a model of differential developmental competence. RESULTS: Using real time PCR, we have analyzed variation of maternal transcripts, in terms of abundance and polyadenylation, during in vitro or in vivo oocyte maturation and in vitro embryo development. Four genes are characterized here for the first time in bovine: ring finger protein 18 (RNF18) and breast cancer anti-estrogen resistance 4 (BCAR4), whose oocyte preferential expression was not previously reported in any species, as well as Maternal embryonic leucine zipper kinase (MELK) and STELLA. We included three known oocyte marker genes (Maternal antigen that embryos require (MATER), Zygote arrest 1 (ZAR1), NACHT, leucine rich repeat and PYD containing 9 (NALP9)). In addition, we selected transcripts previously identified as differentially regulated during maturation, peroxiredoxin 1 and 2 (PRDX1, PRDX2), inhibitor of DNA binding 2 and 3 (ID2, ID3), cyclin B1 (CCNB1), cell division cycle 2 (CDC2), as well as Aurora A (AURKA). Most transcripts underwent a moderate degradation during maturation. But they displayed sharply contrasted deadenylation patterns that account for variations observed previously by DNA array and correlated with the presence of a putative cytoplasmic polyadenylation element in their 3' untranslated region. Similar variations in abundance and polyadenylation status were observed during in vitro maturation or in vivo maturation, except for PRDX1, that appears as a marker of in vivo maturation. Throughout in vitro development, oocyte restricted transcripts were progressively degraded until the morula stage, except for MELK ; and the corresponding genes remained silent after major embryonic genome activation. CONCLUSION: Altogether, our data emphasize the extent of post-transcriptional regulation during oocyte maturation. They do not evidence a general alteration of this phenomenon after in vitro maturation as compared to in vivo maturation, but indicate that some individual messenger RNA can be affected.
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spelling pubmed-22114882008-01-22 Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo Thélie, Aurore Papillier, Pascal Pennetier, Sophie Perreau, Christine Traverso, Juan Martin Uzbekova, Svetlana Mermillod, Pascal Joly, Catherine Humblot, Patrice Dalbiès-Tran, Rozenn BMC Dev Biol Research Article BACKGROUND: In bovine maturing oocytes and cleavage stage embryos, gene expression is mostly controlled at the post-transcriptional level, through degradation and deadenylation/polyadenylation. We have investigated how post transcriptional control of maternal transcripts was affected during in vitro and in vivo maturation, as a model of differential developmental competence. RESULTS: Using real time PCR, we have analyzed variation of maternal transcripts, in terms of abundance and polyadenylation, during in vitro or in vivo oocyte maturation and in vitro embryo development. Four genes are characterized here for the first time in bovine: ring finger protein 18 (RNF18) and breast cancer anti-estrogen resistance 4 (BCAR4), whose oocyte preferential expression was not previously reported in any species, as well as Maternal embryonic leucine zipper kinase (MELK) and STELLA. We included three known oocyte marker genes (Maternal antigen that embryos require (MATER), Zygote arrest 1 (ZAR1), NACHT, leucine rich repeat and PYD containing 9 (NALP9)). In addition, we selected transcripts previously identified as differentially regulated during maturation, peroxiredoxin 1 and 2 (PRDX1, PRDX2), inhibitor of DNA binding 2 and 3 (ID2, ID3), cyclin B1 (CCNB1), cell division cycle 2 (CDC2), as well as Aurora A (AURKA). Most transcripts underwent a moderate degradation during maturation. But they displayed sharply contrasted deadenylation patterns that account for variations observed previously by DNA array and correlated with the presence of a putative cytoplasmic polyadenylation element in their 3' untranslated region. Similar variations in abundance and polyadenylation status were observed during in vitro maturation or in vivo maturation, except for PRDX1, that appears as a marker of in vivo maturation. Throughout in vitro development, oocyte restricted transcripts were progressively degraded until the morula stage, except for MELK ; and the corresponding genes remained silent after major embryonic genome activation. CONCLUSION: Altogether, our data emphasize the extent of post-transcriptional regulation during oocyte maturation. They do not evidence a general alteration of this phenomenon after in vitro maturation as compared to in vivo maturation, but indicate that some individual messenger RNA can be affected. BioMed Central 2007-11-07 /pmc/articles/PMC2211488/ /pubmed/17988387 http://dx.doi.org/10.1186/1471-213X-7-125 Text en Copyright © 2007 Thélie et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Thélie, Aurore
Papillier, Pascal
Pennetier, Sophie
Perreau, Christine
Traverso, Juan Martin
Uzbekova, Svetlana
Mermillod, Pascal
Joly, Catherine
Humblot, Patrice
Dalbiès-Tran, Rozenn
Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title_full Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title_fullStr Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title_full_unstemmed Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title_short Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
title_sort differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211488/
https://www.ncbi.nlm.nih.gov/pubmed/17988387
http://dx.doi.org/10.1186/1471-213X-7-125
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