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Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors

The African trypanosome Trypanosoma brucei, which causes sleeping sickness in humans and Nagana disease in livestock, is spread via blood-sucking Tsetse flies. In the fly's intestine, the trypanosomes survive digestive and trypanocidal environments, proliferate, and translocate into the salivar...

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Autores principales: Nagamune, Kisaburo, Acosta-Serrano, Alvaro, Uemura, Haruki, Brun, Reto, Kunz-Renggli, Christina, Maeda, Yusuke, Ferguson, Michael A.J., Kinoshita, Taroh
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211819/
https://www.ncbi.nlm.nih.gov/pubmed/15136592
http://dx.doi.org/10.1084/jem.20030635
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author Nagamune, Kisaburo
Acosta-Serrano, Alvaro
Uemura, Haruki
Brun, Reto
Kunz-Renggli, Christina
Maeda, Yusuke
Ferguson, Michael A.J.
Kinoshita, Taroh
author_facet Nagamune, Kisaburo
Acosta-Serrano, Alvaro
Uemura, Haruki
Brun, Reto
Kunz-Renggli, Christina
Maeda, Yusuke
Ferguson, Michael A.J.
Kinoshita, Taroh
author_sort Nagamune, Kisaburo
collection PubMed
description The African trypanosome Trypanosoma brucei, which causes sleeping sickness in humans and Nagana disease in livestock, is spread via blood-sucking Tsetse flies. In the fly's intestine, the trypanosomes survive digestive and trypanocidal environments, proliferate, and translocate into the salivary gland, where they become infectious to the next mammalian host. Here, we show that for successful survival in Tsetse flies, the trypanosomes use trans-sialidase to transfer sialic acids that they cannot synthesize from host's glycoconjugates to the glycosylphosphatidylinositols (GPIs), which are abundantly expressed on their surface. Trypanosomes lacking sialic acids due to a defective generation of GPI-anchored trans-sialidase could not survive in the intestine, but regained the ability to survive when sialylated by means of soluble trans-sialidase. Thus, surface sialic acids appear to protect the parasites from the digestive and trypanocidal environments in the midgut of Tsetse flies.
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spelling pubmed-22118192008-03-11 Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors Nagamune, Kisaburo Acosta-Serrano, Alvaro Uemura, Haruki Brun, Reto Kunz-Renggli, Christina Maeda, Yusuke Ferguson, Michael A.J. Kinoshita, Taroh J Exp Med Brief Definitive Report The African trypanosome Trypanosoma brucei, which causes sleeping sickness in humans and Nagana disease in livestock, is spread via blood-sucking Tsetse flies. In the fly's intestine, the trypanosomes survive digestive and trypanocidal environments, proliferate, and translocate into the salivary gland, where they become infectious to the next mammalian host. Here, we show that for successful survival in Tsetse flies, the trypanosomes use trans-sialidase to transfer sialic acids that they cannot synthesize from host's glycoconjugates to the glycosylphosphatidylinositols (GPIs), which are abundantly expressed on their surface. Trypanosomes lacking sialic acids due to a defective generation of GPI-anchored trans-sialidase could not survive in the intestine, but regained the ability to survive when sialylated by means of soluble trans-sialidase. Thus, surface sialic acids appear to protect the parasites from the digestive and trypanocidal environments in the midgut of Tsetse flies. The Rockefeller University Press 2004-05-17 /pmc/articles/PMC2211819/ /pubmed/15136592 http://dx.doi.org/10.1084/jem.20030635 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Nagamune, Kisaburo
Acosta-Serrano, Alvaro
Uemura, Haruki
Brun, Reto
Kunz-Renggli, Christina
Maeda, Yusuke
Ferguson, Michael A.J.
Kinoshita, Taroh
Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title_full Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title_fullStr Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title_full_unstemmed Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title_short Surface Sialic Acids Taken from the Host Allow Trypanosome Survival in Tsetse Fly Vectors
title_sort surface sialic acids taken from the host allow trypanosome survival in tsetse fly vectors
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211819/
https://www.ncbi.nlm.nih.gov/pubmed/15136592
http://dx.doi.org/10.1084/jem.20030635
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