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Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes

Cadherins are integral membrane proteins expressed in tissue-restricted patterns that mediate homophilic intercellular adhesion. During development, they orchestrate tissue morphogenesis and, in the adult, they determine tissue integrity and architecture. The synovial lining is a condensation of fib...

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Autores principales: Valencia, Xavier, Higgins, Jonathan M.G., Kiener, Hans P., Lee, David M., Podrebarac, Theresa A., Dascher, Christopher C., Watts, Gerald F.M., Mizoguchi, Emiko, Simmons, Barry, Patel, Dhavalkumar D., Bhan, Atul K., Brenner, Michael B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211995/
https://www.ncbi.nlm.nih.gov/pubmed/15611293
http://dx.doi.org/10.1084/jem.20041545
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author Valencia, Xavier
Higgins, Jonathan M.G.
Kiener, Hans P.
Lee, David M.
Podrebarac, Theresa A.
Dascher, Christopher C.
Watts, Gerald F.M.
Mizoguchi, Emiko
Simmons, Barry
Patel, Dhavalkumar D.
Bhan, Atul K.
Brenner, Michael B.
author_facet Valencia, Xavier
Higgins, Jonathan M.G.
Kiener, Hans P.
Lee, David M.
Podrebarac, Theresa A.
Dascher, Christopher C.
Watts, Gerald F.M.
Mizoguchi, Emiko
Simmons, Barry
Patel, Dhavalkumar D.
Bhan, Atul K.
Brenner, Michael B.
author_sort Valencia, Xavier
collection PubMed
description Cadherins are integral membrane proteins expressed in tissue-restricted patterns that mediate homophilic intercellular adhesion. During development, they orchestrate tissue morphogenesis and, in the adult, they determine tissue integrity and architecture. The synovial lining is a condensation of fibroblast-like synoviocytes (FLS) and macrophages one to three cells thick. These cells are embedded within the extracellular matrix, but the structure is neither an epithelium nor an endothelium. Previously, the basis for organization of the synovium into a tissue was unknown. Here, we cloned cadherin-11 from human rheumatoid arthritis (RA)-derived FLS. We developed L cell transfectants expressing cadherin-11, cadherin-11 fusion proteins, and anti–cadherin-11 mAb. Cadherin-11 was found to be expressed mainly in the synovial lining by immunohistologic staining of human synovium. FLS adhered to cadherin-11–Fc, and transfection of cadherin-11 conferred the formation of tissue-like sheets and lining-like structures upon fibroblasts in vitro. These findings support a key role for cadherin-11 in the specific adhesion of FLS and in synovial tissue organization and behavior in health and RA.
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spelling pubmed-22119952008-03-11 Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes Valencia, Xavier Higgins, Jonathan M.G. Kiener, Hans P. Lee, David M. Podrebarac, Theresa A. Dascher, Christopher C. Watts, Gerald F.M. Mizoguchi, Emiko Simmons, Barry Patel, Dhavalkumar D. Bhan, Atul K. Brenner, Michael B. J Exp Med Brief Definitive Report Cadherins are integral membrane proteins expressed in tissue-restricted patterns that mediate homophilic intercellular adhesion. During development, they orchestrate tissue morphogenesis and, in the adult, they determine tissue integrity and architecture. The synovial lining is a condensation of fibroblast-like synoviocytes (FLS) and macrophages one to three cells thick. These cells are embedded within the extracellular matrix, but the structure is neither an epithelium nor an endothelium. Previously, the basis for organization of the synovium into a tissue was unknown. Here, we cloned cadherin-11 from human rheumatoid arthritis (RA)-derived FLS. We developed L cell transfectants expressing cadherin-11, cadherin-11 fusion proteins, and anti–cadherin-11 mAb. Cadherin-11 was found to be expressed mainly in the synovial lining by immunohistologic staining of human synovium. FLS adhered to cadherin-11–Fc, and transfection of cadherin-11 conferred the formation of tissue-like sheets and lining-like structures upon fibroblasts in vitro. These findings support a key role for cadherin-11 in the specific adhesion of FLS and in synovial tissue organization and behavior in health and RA. The Rockefeller University Press 2004-12-20 /pmc/articles/PMC2211995/ /pubmed/15611293 http://dx.doi.org/10.1084/jem.20041545 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Valencia, Xavier
Higgins, Jonathan M.G.
Kiener, Hans P.
Lee, David M.
Podrebarac, Theresa A.
Dascher, Christopher C.
Watts, Gerald F.M.
Mizoguchi, Emiko
Simmons, Barry
Patel, Dhavalkumar D.
Bhan, Atul K.
Brenner, Michael B.
Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title_full Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title_fullStr Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title_full_unstemmed Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title_short Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
title_sort cadherin-11 provides specific cellular adhesion between fibroblast-like synoviocytes
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211995/
https://www.ncbi.nlm.nih.gov/pubmed/15611293
http://dx.doi.org/10.1084/jem.20041545
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