THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD

A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently elut...

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Detalles Bibliográficos
Autores principales: Skeggs, Leonard T., Kahn, Joseph R., Shumway, Norman P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1952
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212071/
https://www.ncbi.nlm.nih.gov/pubmed/14927788
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author Skeggs, Leonard T.
Kahn, Joseph R.
Shumway, Norman P.
author_facet Skeggs, Leonard T.
Kahn, Joseph R.
Shumway, Norman P.
author_sort Skeggs, Leonard T.
collection PubMed
description A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently eluted with dilute ethanol. The eluate is then evaporated to dryness, dissolved in water, and assayed by intravenous injection into rats.
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spelling pubmed-22120712008-04-17 THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD Skeggs, Leonard T. Kahn, Joseph R. Shumway, Norman P. J Exp Med Article A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently eluted with dilute ethanol. The eluate is then evaporated to dryness, dissolved in water, and assayed by intravenous injection into rats. The Rockefeller University Press 1952-02-29 /pmc/articles/PMC2212071/ /pubmed/14927788 Text en Copyright © Copyright, 1952, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Skeggs, Leonard T.
Kahn, Joseph R.
Shumway, Norman P.
THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title_full THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title_fullStr THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title_full_unstemmed THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title_short THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD
title_sort isolation and assay of hypertensin from blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212071/
https://www.ncbi.nlm.nih.gov/pubmed/14927788
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