Cargando…

Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics

Polymorphonuclear granulocytes (PMNs) are thought to fulfill their role in host defense primarily via phagocytosis and release of cytotoxic compounds and to be inefficient in antigen presentation and stimulation of specific T cells. Dendritic cells (DCs), in contrast, are potent antigen-presenting c...

Descripción completa

Detalles Bibliográficos
Autores principales: Oehler, Leopold, Majdic, Otto, Pickl, Winfried F., Stöckl, Johannes, Riedl, Elisabeth, Drach, Johannes, Rappersberger, Klemens, Geissler, Klaus, Knapp, Walter
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212207/
https://www.ncbi.nlm.nih.gov/pubmed/9529318
_version_ 1782148649575251968
author Oehler, Leopold
Majdic, Otto
Pickl, Winfried F.
Stöckl, Johannes
Riedl, Elisabeth
Drach, Johannes
Rappersberger, Klemens
Geissler, Klaus
Knapp, Walter
author_facet Oehler, Leopold
Majdic, Otto
Pickl, Winfried F.
Stöckl, Johannes
Riedl, Elisabeth
Drach, Johannes
Rappersberger, Klemens
Geissler, Klaus
Knapp, Walter
author_sort Oehler, Leopold
collection PubMed
description Polymorphonuclear granulocytes (PMNs) are thought to fulfill their role in host defense primarily via phagocytosis and release of cytotoxic compounds and to be inefficient in antigen presentation and stimulation of specific T cells. Dendritic cells (DCs), in contrast, are potent antigen-presenting cells with the unique capacity to initiate primary immune responses. We demonstrate here that highly purified lactoferrin-positive immediate precursors of end-stage neutrophilic PMN (PMNp) can be reverted in their functional maturation program and driven to acquire characteristic DC features. Upon culture with the cytokine combination granulocyte/macrophage colony-stimulating factor plus interleukin 4 plus tumor necrosis factor α, they develop DC morphology and acquire molecular features characteristic for DCs. These molecular changes include neo-expression of the DC-associated surface molecules cluster of differentiation (CD)1a, CD1b, CD1c, human leukocyte antigen (HLA)-DR, HLA-DQ, CD80, CD86, CD40, CD54, and CD5, and downregulation of CD15 and CD65s. Additional stimulation with CD40 ligand induces also expression of CD83 and upregulates CD80, CD86, and HLA-DR. The neutrophil-derived DCs are potent T cell stimulators in allogeneic, as well as autologous, mixed lymphocyte reactions (MLRs), whereas freshly isolated neutrophils are completely unable to do so. In addition, neutrophil-derived DCs are at least 10,000 times more efficient in presenting soluble antigen to autologous T cells when compared to freshly isolated monocytes. Also, in functional terms, these neutrophil-derived DCs thus closely resemble “classical” DC populations.
format Text
id pubmed-2212207
institution National Center for Biotechnology Information
language English
publishDate 1998
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22122072008-04-16 Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics Oehler, Leopold Majdic, Otto Pickl, Winfried F. Stöckl, Johannes Riedl, Elisabeth Drach, Johannes Rappersberger, Klemens Geissler, Klaus Knapp, Walter J Exp Med Article Polymorphonuclear granulocytes (PMNs) are thought to fulfill their role in host defense primarily via phagocytosis and release of cytotoxic compounds and to be inefficient in antigen presentation and stimulation of specific T cells. Dendritic cells (DCs), in contrast, are potent antigen-presenting cells with the unique capacity to initiate primary immune responses. We demonstrate here that highly purified lactoferrin-positive immediate precursors of end-stage neutrophilic PMN (PMNp) can be reverted in their functional maturation program and driven to acquire characteristic DC features. Upon culture with the cytokine combination granulocyte/macrophage colony-stimulating factor plus interleukin 4 plus tumor necrosis factor α, they develop DC morphology and acquire molecular features characteristic for DCs. These molecular changes include neo-expression of the DC-associated surface molecules cluster of differentiation (CD)1a, CD1b, CD1c, human leukocyte antigen (HLA)-DR, HLA-DQ, CD80, CD86, CD40, CD54, and CD5, and downregulation of CD15 and CD65s. Additional stimulation with CD40 ligand induces also expression of CD83 and upregulates CD80, CD86, and HLA-DR. The neutrophil-derived DCs are potent T cell stimulators in allogeneic, as well as autologous, mixed lymphocyte reactions (MLRs), whereas freshly isolated neutrophils are completely unable to do so. In addition, neutrophil-derived DCs are at least 10,000 times more efficient in presenting soluble antigen to autologous T cells when compared to freshly isolated monocytes. Also, in functional terms, these neutrophil-derived DCs thus closely resemble “classical” DC populations. The Rockefeller University Press 1998-04-06 /pmc/articles/PMC2212207/ /pubmed/9529318 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Oehler, Leopold
Majdic, Otto
Pickl, Winfried F.
Stöckl, Johannes
Riedl, Elisabeth
Drach, Johannes
Rappersberger, Klemens
Geissler, Klaus
Knapp, Walter
Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title_full Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title_fullStr Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title_full_unstemmed Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title_short Neutrophil Granulocyte–committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
title_sort neutrophil granulocyte–committed cells can be driven to acquire dendritic cell characteristics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212207/
https://www.ncbi.nlm.nih.gov/pubmed/9529318
work_keys_str_mv AT oehlerleopold neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT majdicotto neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT picklwinfriedf neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT stockljohannes neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT riedlelisabeth neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT drachjohannes neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT rappersbergerklemens neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT geisslerklaus neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics
AT knappwalter neutrophilgranulocytecommittedcellscanbedriventoacquiredendriticcellcharacteristics