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Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells

Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase Cγ-1 and Grb2. Microsequencing of peptides derived from purified rat pp36 protein led to the cloning, in rat and man, of cDNA e...

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Autores principales: Weber, John R., Ørstavik, Sigurd, Torgersen, Knut Martin, Danbolt, Niels Christian, Berg, Siri F., Ryan, James C., Taskén, Kjetil, Imboden, John B., Vaage, John T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212210/
https://www.ncbi.nlm.nih.gov/pubmed/9529333
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author Weber, John R.
Ørstavik, Sigurd
Torgersen, Knut Martin
Danbolt, Niels Christian
Berg, Siri F.
Ryan, James C.
Taskén, Kjetil
Imboden, John B.
Vaage, John T.
author_facet Weber, John R.
Ørstavik, Sigurd
Torgersen, Knut Martin
Danbolt, Niels Christian
Berg, Siri F.
Ryan, James C.
Taskén, Kjetil
Imboden, John B.
Vaage, John T.
author_sort Weber, John R.
collection PubMed
description Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase Cγ-1 and Grb2. Microsequencing of peptides derived from purified rat pp36 protein led to the cloning, in rat and man, of cDNA encoding a T- and NK cell–specific protein with several putative Src homology 2 domain–binding motifs. A rabbit antiserum directed against a peptide sequence from the cloned rat molecule recognized tyrosine phosphorylated pp36 from pervanadate-treated rat thymocytes. When expressed in 293T human fibroblast cells and tyrosine-phosphorylated, pp36 associated with phospholipase Cγ-1 and Grb2. Studies with GST–Grb2 fusion proteins demonstrated that the association was specific for the Src homology 2 domain of Grb-2. Molecular cloning of the gene encoding pp36 should facilitate studies examining the role of this adaptor protein in proximal signaling events during T and NK cell activation.
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spelling pubmed-22122102008-04-16 Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells Weber, John R. Ørstavik, Sigurd Torgersen, Knut Martin Danbolt, Niels Christian Berg, Siri F. Ryan, James C. Taskén, Kjetil Imboden, John B. Vaage, John T. J Exp Med Brief Definitive Report Activation of T and natural killer (NK) cells leads to the tyrosine phosphorylation of pp36 and to its association with several signaling molecules, including phospholipase Cγ-1 and Grb2. Microsequencing of peptides derived from purified rat pp36 protein led to the cloning, in rat and man, of cDNA encoding a T- and NK cell–specific protein with several putative Src homology 2 domain–binding motifs. A rabbit antiserum directed against a peptide sequence from the cloned rat molecule recognized tyrosine phosphorylated pp36 from pervanadate-treated rat thymocytes. When expressed in 293T human fibroblast cells and tyrosine-phosphorylated, pp36 associated with phospholipase Cγ-1 and Grb2. Studies with GST–Grb2 fusion proteins demonstrated that the association was specific for the Src homology 2 domain of Grb-2. Molecular cloning of the gene encoding pp36 should facilitate studies examining the role of this adaptor protein in proximal signaling events during T and NK cell activation. The Rockefeller University Press 1998-04-06 /pmc/articles/PMC2212210/ /pubmed/9529333 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Weber, John R.
Ørstavik, Sigurd
Torgersen, Knut Martin
Danbolt, Niels Christian
Berg, Siri F.
Ryan, James C.
Taskén, Kjetil
Imboden, John B.
Vaage, John T.
Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title_full Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title_fullStr Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title_full_unstemmed Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title_short Molecular Cloning of the cDNA Encoding pp36, a Tyrosine-phosphorylated Adaptor Protein Selectively Expressed by T Cells and Natural Killer Cells
title_sort molecular cloning of the cdna encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by t cells and natural killer cells
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212210/
https://www.ncbi.nlm.nih.gov/pubmed/9529333
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