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The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles
The transfer of lymphocytes into severe combined immunodeficiency (SCID) mice induces a series of histological changes in the spleen, including the appearance of mature follicular dendritic cells (FDCs). Studies were undertaken to clarify the role of lymphotoxin (LT) in this process. The results sho...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212214/ https://www.ncbi.nlm.nih.gov/pubmed/9529316 |
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author | Gonzalez, Mercedes Mackay, Fabienne Browning, Jeffrey L. Kosco-Vilbois, Marie H. Noelle, Randolph J. |
author_facet | Gonzalez, Mercedes Mackay, Fabienne Browning, Jeffrey L. Kosco-Vilbois, Marie H. Noelle, Randolph J. |
author_sort | Gonzalez, Mercedes |
collection | PubMed |
description | The transfer of lymphocytes into severe combined immunodeficiency (SCID) mice induces a series of histological changes in the spleen, including the appearance of mature follicular dendritic cells (FDCs). Studies were undertaken to clarify the role of lymphotoxin (LT) in this process. The results show that SCID mice have a small and partially differentiated white pulp containing marginal zone and interdigitating dendritic cells, but lacking FDCs. Transferred spleen cells can segregate into T and B cell areas shortly after their injection to SCID mice. This ability is dependent on signaling through LT-β receptor (LT-βR), since blocking ligand–receptor interaction in recipient SCID mice ablates the capacity of the transferred cells to segregate. A week after lymphocyte transfer, host-derived FDCs appeared in the reconstituted SCID mice. This induction of FDCs is dependent on LT-βR signaling by B cells since LT-α(−/−) B cells are incapable of inducing development of FDCs in SCID mice, even after cotransfer of LT-α(+/+) T cells. Therefore, LT plays at least two discrete roles in splenic organization. First, it appears that LT induces the differentiation of the white pulp to create sites for lymphocyte segregation. Second, LT expression by B cells drives the maturation of FDCs and the organization of B cell follicles. |
format | Text |
id | pubmed-2212214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22122142008-04-16 The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles Gonzalez, Mercedes Mackay, Fabienne Browning, Jeffrey L. Kosco-Vilbois, Marie H. Noelle, Randolph J. J Exp Med Article The transfer of lymphocytes into severe combined immunodeficiency (SCID) mice induces a series of histological changes in the spleen, including the appearance of mature follicular dendritic cells (FDCs). Studies were undertaken to clarify the role of lymphotoxin (LT) in this process. The results show that SCID mice have a small and partially differentiated white pulp containing marginal zone and interdigitating dendritic cells, but lacking FDCs. Transferred spleen cells can segregate into T and B cell areas shortly after their injection to SCID mice. This ability is dependent on signaling through LT-β receptor (LT-βR), since blocking ligand–receptor interaction in recipient SCID mice ablates the capacity of the transferred cells to segregate. A week after lymphocyte transfer, host-derived FDCs appeared in the reconstituted SCID mice. This induction of FDCs is dependent on LT-βR signaling by B cells since LT-α(−/−) B cells are incapable of inducing development of FDCs in SCID mice, even after cotransfer of LT-α(+/+) T cells. Therefore, LT plays at least two discrete roles in splenic organization. First, it appears that LT induces the differentiation of the white pulp to create sites for lymphocyte segregation. Second, LT expression by B cells drives the maturation of FDCs and the organization of B cell follicles. The Rockefeller University Press 1998-04-06 /pmc/articles/PMC2212214/ /pubmed/9529316 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Gonzalez, Mercedes Mackay, Fabienne Browning, Jeffrey L. Kosco-Vilbois, Marie H. Noelle, Randolph J. The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title | The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title_full | The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title_fullStr | The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title_full_unstemmed | The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title_short | The Sequential Role of Lymphotoxin and B Cells in the Development of Splenic Follicles |
title_sort | sequential role of lymphotoxin and b cells in the development of splenic follicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212214/ https://www.ncbi.nlm.nih.gov/pubmed/9529316 |
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