Cargando…
Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex
A tetrameric recombinant major histocompatibility complex (MHC) class I–peptide complex was used as a staining reagent in flow cytometric analyses to quantitate and define the phenotype of Gag-specific cytotoxic T lymphocytes (CTLs) in the peripheral blood of simian immunodeficiency virus macaque (S...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1998
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212269/ https://www.ncbi.nlm.nih.gov/pubmed/9565630 |
_version_ | 1782148659669893120 |
---|---|
author | Kuroda, Marcelo J. Schmitz, Jörn E. Barouch, Dan H. Craiu, Abie Allen, Todd M. Sette, Alessandro Watkins, David I. Forman, Meryl A. Letvin, Norman L. |
author_facet | Kuroda, Marcelo J. Schmitz, Jörn E. Barouch, Dan H. Craiu, Abie Allen, Todd M. Sette, Alessandro Watkins, David I. Forman, Meryl A. Letvin, Norman L. |
author_sort | Kuroda, Marcelo J. |
collection | PubMed |
description | A tetrameric recombinant major histocompatibility complex (MHC) class I–peptide complex was used as a staining reagent in flow cytometric analyses to quantitate and define the phenotype of Gag-specific cytotoxic T lymphocytes (CTLs) in the peripheral blood of simian immunodeficiency virus macaque (SIVmac)-infected rhesus monkeys. The heavy chain of the rhesus monkey MHC class I molecule Mamu-A*01 and β(2)-microglobulin were refolded in the presence of an SIVmac Gag synthetic peptide (p11C, C–M) representing the optimal nine–amino acid peptide of Mamu-A*01–restricted predominant CTL epitope to create a tetrameric Mamu-A*01/p11C, C–M complex. Tetrameric Mamu-A*01/p11C, C–M complex bound to T cells of SIVmac-infected, Mamu-A*01(+), but not uninfected, Mamu-A*01(+), or infected, Mamu-A*01(−) rhesus monkeys. Specific staining of peripheral blood mononuclear cells (PBMC) from SIVmac-infected, Mamu-A*01(+) rhesus monkeys was only found in the cluster of differentiation (CD)8α/β(+) T lymphocyte subset and the percentage of CD8α/β(+) T cells in the peripheral blood of four SIVmac-infected, Mamu-A*01(+) rhesus monkeys staining with this complex ranged from 0.7 to 10.3%. Importantly, functional SIVmac Gag p11C-specific CTL activity was seen in sorted and expanded tetrameric Mamu-A*01/p11C, C–M complex–binding, but not nonbinding, CD8α/β(+) T cells. Furthermore, the percentage of CD8α/β(+) T cells binding this tetrameric Mamu-A*01/p11C, C–M complex correlated well with p11C-specific cytotoxic activity as measured in both bulk and limiting dilution effector frequency assays. Finally, phenotypic characterization of the cells binding this tetrameric complex indicated that this lymphocyte population is heterogeneous. These studies indicate the power of this approach for examining virus-specific CTLs in in vivo settings. |
format | Text |
id | pubmed-2212269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22122692008-04-16 Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex Kuroda, Marcelo J. Schmitz, Jörn E. Barouch, Dan H. Craiu, Abie Allen, Todd M. Sette, Alessandro Watkins, David I. Forman, Meryl A. Letvin, Norman L. J Exp Med Article A tetrameric recombinant major histocompatibility complex (MHC) class I–peptide complex was used as a staining reagent in flow cytometric analyses to quantitate and define the phenotype of Gag-specific cytotoxic T lymphocytes (CTLs) in the peripheral blood of simian immunodeficiency virus macaque (SIVmac)-infected rhesus monkeys. The heavy chain of the rhesus monkey MHC class I molecule Mamu-A*01 and β(2)-microglobulin were refolded in the presence of an SIVmac Gag synthetic peptide (p11C, C–M) representing the optimal nine–amino acid peptide of Mamu-A*01–restricted predominant CTL epitope to create a tetrameric Mamu-A*01/p11C, C–M complex. Tetrameric Mamu-A*01/p11C, C–M complex bound to T cells of SIVmac-infected, Mamu-A*01(+), but not uninfected, Mamu-A*01(+), or infected, Mamu-A*01(−) rhesus monkeys. Specific staining of peripheral blood mononuclear cells (PBMC) from SIVmac-infected, Mamu-A*01(+) rhesus monkeys was only found in the cluster of differentiation (CD)8α/β(+) T lymphocyte subset and the percentage of CD8α/β(+) T cells in the peripheral blood of four SIVmac-infected, Mamu-A*01(+) rhesus monkeys staining with this complex ranged from 0.7 to 10.3%. Importantly, functional SIVmac Gag p11C-specific CTL activity was seen in sorted and expanded tetrameric Mamu-A*01/p11C, C–M complex–binding, but not nonbinding, CD8α/β(+) T cells. Furthermore, the percentage of CD8α/β(+) T cells binding this tetrameric Mamu-A*01/p11C, C–M complex correlated well with p11C-specific cytotoxic activity as measured in both bulk and limiting dilution effector frequency assays. Finally, phenotypic characterization of the cells binding this tetrameric complex indicated that this lymphocyte population is heterogeneous. These studies indicate the power of this approach for examining virus-specific CTLs in in vivo settings. The Rockefeller University Press 1998-05-04 /pmc/articles/PMC2212269/ /pubmed/9565630 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kuroda, Marcelo J. Schmitz, Jörn E. Barouch, Dan H. Craiu, Abie Allen, Todd M. Sette, Alessandro Watkins, David I. Forman, Meryl A. Letvin, Norman L. Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title | Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title_full | Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title_fullStr | Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title_full_unstemmed | Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title_short | Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex |
title_sort | analysis of gag-specific cytotoxic t lymphocytes in simian immunodeficiency virus–infected rhesus monkeys by cell staining with a tetrameric major histocompatibility complex class i–peptide complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212269/ https://www.ncbi.nlm.nih.gov/pubmed/9565630 |
work_keys_str_mv | AT kurodamarceloj analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT schmitzjorne analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT barouchdanh analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT craiuabie analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT allentoddm analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT settealessandro analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT watkinsdavidi analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT formanmeryla analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex AT letvinnormanl analysisofgagspecificcytotoxictlymphocytesinsimianimmunodeficiencyvirusinfectedrhesusmonkeysbycellstainingwithatetramericmajorhistocompatibilitycomplexclassipeptidecomplex |