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Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex

The positive selection of CD4(+) T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single p...

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Detalles Bibliográficos
Autores principales: Gapin, Laurent, Fukui, Yoshinori, Kanellopoulos, Jean, Sano, Tetsuro, Casrouge, Armanda, Malier, Vanessa, Beaudoing, Emmanuel, Gautheret, Daniel, Claverie, Jean-Michel, Sasazuki, Takehiko, Kourilsky, Philippe
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212317/
https://www.ncbi.nlm.nih.gov/pubmed/9607927
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author Gapin, Laurent
Fukui, Yoshinori
Kanellopoulos, Jean
Sano, Tetsuro
Casrouge, Armanda
Malier, Vanessa
Beaudoing, Emmanuel
Gautheret, Daniel
Claverie, Jean-Michel
Sasazuki, Takehiko
Kourilsky, Philippe
author_facet Gapin, Laurent
Fukui, Yoshinori
Kanellopoulos, Jean
Sano, Tetsuro
Casrouge, Armanda
Malier, Vanessa
Beaudoing, Emmanuel
Gautheret, Daniel
Claverie, Jean-Michel
Sasazuki, Takehiko
Kourilsky, Philippe
author_sort Gapin, Laurent
collection PubMed
description The positive selection of CD4(+) T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide–MHC class II complex positively select significant numbers of diverse CD4(+) T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide–MHC class II complex positively selects at least 10(5) different Vβ rearrangements. Our data yield a first evaluation of the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68–I-A(b) complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.
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spelling pubmed-22123172008-04-16 Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex Gapin, Laurent Fukui, Yoshinori Kanellopoulos, Jean Sano, Tetsuro Casrouge, Armanda Malier, Vanessa Beaudoing, Emmanuel Gautheret, Daniel Claverie, Jean-Michel Sasazuki, Takehiko Kourilsky, Philippe J Exp Med Article The positive selection of CD4(+) T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide–MHC class II complex positively select significant numbers of diverse CD4(+) T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide–MHC class II complex positively selects at least 10(5) different Vβ rearrangements. Our data yield a first evaluation of the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68–I-A(b) complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide. The Rockefeller University Press 1998-06-01 /pmc/articles/PMC2212317/ /pubmed/9607927 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gapin, Laurent
Fukui, Yoshinori
Kanellopoulos, Jean
Sano, Tetsuro
Casrouge, Armanda
Malier, Vanessa
Beaudoing, Emmanuel
Gautheret, Daniel
Claverie, Jean-Michel
Sasazuki, Takehiko
Kourilsky, Philippe
Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title_full Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title_fullStr Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title_full_unstemmed Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title_short Quantitative Analysis of the T Cell Repertoire Selected by a Single Peptide–Major Histocompatibility Complex
title_sort quantitative analysis of the t cell repertoire selected by a single peptide–major histocompatibility complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212317/
https://www.ncbi.nlm.nih.gov/pubmed/9607927
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