2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48

2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely bloc...

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Autores principales: Brown, Marion H., Boles, Kent, Anton van der Merwe, P., Kumar, Vinay, Mathew, Porunelloor A., Neil Barclay, A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212392/
https://www.ncbi.nlm.nih.gov/pubmed/9841922
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author Brown, Marion H.
Boles, Kent
Anton van der Merwe, P.
Kumar, Vinay
Mathew, Porunelloor A.
Neil Barclay, A.
author_facet Brown, Marion H.
Boles, Kent
Anton van der Merwe, P.
Kumar, Vinay
Mathew, Porunelloor A.
Neil Barclay, A.
author_sort Brown, Marion H.
collection PubMed
description 2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (K (d )≈ 16 μM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (K (d )≈ 8 μM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation.
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spelling pubmed-22123922008-04-16 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48 Brown, Marion H. Boles, Kent Anton van der Merwe, P. Kumar, Vinay Mathew, Porunelloor A. Neil Barclay, A. J Exp Med Articles 2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (K (d )≈ 16 μM at 37°C) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (K (d )≈ 8 μM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation. The Rockefeller University Press 1998-12-07 /pmc/articles/PMC2212392/ /pubmed/9841922 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Brown, Marion H.
Boles, Kent
Anton van der Merwe, P.
Kumar, Vinay
Mathew, Porunelloor A.
Neil Barclay, A.
2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title_full 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title_fullStr 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title_full_unstemmed 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title_short 2B4, the Natural Killer and T Cell Immunoglobulin Superfamily Surface Protein, Is a Ligand for CD48
title_sort 2b4, the natural killer and t cell immunoglobulin superfamily surface protein, is a ligand for cd48
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212392/
https://www.ncbi.nlm.nih.gov/pubmed/9841922
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