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The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit

The most primitive engrafting hematopoietic stem cell has been assumed to have a fixed phenotype, with changes in engraftment and renewal potential occurring in a stepwise irreversible fashion linked with differentiation. Recent work shows that in vitro cytokine stimulation of murine marrow cells in...

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Autores principales: Habibian, Houri K., Peters, Stefan O., Hsieh, C.C., Wuu, Joanne, Vergilis, Kristin, Grimaldi, Christina I., Reilly, Judith, Carlson, Jane E., Frimberger, Angela E., Stewart, F.M., Quesenberry, Peter J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212441/
https://www.ncbi.nlm.nih.gov/pubmed/9670051
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author Habibian, Houri K.
Peters, Stefan O.
Hsieh, C.C.
Wuu, Joanne
Vergilis, Kristin
Grimaldi, Christina I.
Reilly, Judith
Carlson, Jane E.
Frimberger, Angela E.
Stewart, F.M.
Quesenberry, Peter J.
author_facet Habibian, Houri K.
Peters, Stefan O.
Hsieh, C.C.
Wuu, Joanne
Vergilis, Kristin
Grimaldi, Christina I.
Reilly, Judith
Carlson, Jane E.
Frimberger, Angela E.
Stewart, F.M.
Quesenberry, Peter J.
author_sort Habibian, Houri K.
collection PubMed
description The most primitive engrafting hematopoietic stem cell has been assumed to have a fixed phenotype, with changes in engraftment and renewal potential occurring in a stepwise irreversible fashion linked with differentiation. Recent work shows that in vitro cytokine stimulation of murine marrow cells induces cell cycle transit of primitive stem cells, taking 40 h for progression from G(0) to mitosis and 12 h for subsequent doublings. At 48 h of culture, progenitors are expanded, but stem cell engraftment is markedly diminished. We have investigated whether this effect on engraftment was an irreversible step or a reversible plastic feature correlated with cell cycle progression. Long-term engraftment (2 and 6 mo) of male BALB/c marrow cells exposed in vitro to interleukin (IL)-3, IL-6, IL-11, and steel factor was assessed at 2–4-h intervals of culture over 24–48 h using irradiated female hosts; the engraftment phenotype showed marked fluctuations over 2–4-h intervals, with engraftment nadirs occurring in late S and early G(2). These data show that early stem cell regulation is cell cycle based, and have critical implications for strategies for stem cell expansion and engraftment or gene therapy, since position in cell cycle will determine whether effective engraftment occurs in either setting.
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spelling pubmed-22124412008-04-16 The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit Habibian, Houri K. Peters, Stefan O. Hsieh, C.C. Wuu, Joanne Vergilis, Kristin Grimaldi, Christina I. Reilly, Judith Carlson, Jane E. Frimberger, Angela E. Stewart, F.M. Quesenberry, Peter J. J Exp Med Brief Definitive Reports The most primitive engrafting hematopoietic stem cell has been assumed to have a fixed phenotype, with changes in engraftment and renewal potential occurring in a stepwise irreversible fashion linked with differentiation. Recent work shows that in vitro cytokine stimulation of murine marrow cells induces cell cycle transit of primitive stem cells, taking 40 h for progression from G(0) to mitosis and 12 h for subsequent doublings. At 48 h of culture, progenitors are expanded, but stem cell engraftment is markedly diminished. We have investigated whether this effect on engraftment was an irreversible step or a reversible plastic feature correlated with cell cycle progression. Long-term engraftment (2 and 6 mo) of male BALB/c marrow cells exposed in vitro to interleukin (IL)-3, IL-6, IL-11, and steel factor was assessed at 2–4-h intervals of culture over 24–48 h using irradiated female hosts; the engraftment phenotype showed marked fluctuations over 2–4-h intervals, with engraftment nadirs occurring in late S and early G(2). These data show that early stem cell regulation is cell cycle based, and have critical implications for strategies for stem cell expansion and engraftment or gene therapy, since position in cell cycle will determine whether effective engraftment occurs in either setting. The Rockefeller University Press 1998-07-20 /pmc/articles/PMC2212441/ /pubmed/9670051 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Habibian, Houri K.
Peters, Stefan O.
Hsieh, C.C.
Wuu, Joanne
Vergilis, Kristin
Grimaldi, Christina I.
Reilly, Judith
Carlson, Jane E.
Frimberger, Angela E.
Stewart, F.M.
Quesenberry, Peter J.
The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title_full The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title_fullStr The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title_full_unstemmed The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title_short The Fluctuating Phenotype of the Lymphohematopoietic Stem Cell with Cell Cycle Transit
title_sort fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212441/
https://www.ncbi.nlm.nih.gov/pubmed/9670051
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