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Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development
The B lymphocyte–induced maturation protein (Blimp-1) upregulates the expression of syndecan-1 and J chain and represses that of c-myc. We have transfected Blimp-1 into two sublines of the BCL1 B cell lymphoma that represent distinct stages of B cell development in secondary lymphoid tissues. After...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212483/ https://www.ncbi.nlm.nih.gov/pubmed/9687529 |
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author | Messika, Eric J. Lu, Peter S. Sung, Yen-Jen Yao, Tony Chi, Jen-Tsan Chien, Yueh-hsiu Davis, Mark M. |
author_facet | Messika, Eric J. Lu, Peter S. Sung, Yen-Jen Yao, Tony Chi, Jen-Tsan Chien, Yueh-hsiu Davis, Mark M. |
author_sort | Messika, Eric J. |
collection | PubMed |
description | The B lymphocyte–induced maturation protein (Blimp-1) upregulates the expression of syndecan-1 and J chain and represses that of c-myc. We have transfected Blimp-1 into two sublines of the BCL1 B cell lymphoma that represent distinct stages of B cell development in secondary lymphoid tissues. After interleukin (IL)-2 and IL-5 stimulation, the BCL1 3B3 cells differentiate into centrocyte-like cells, whereas the BCL1 5B1b cells blast and appear to be blocked at the centroblast stage. This blasting effect and the increase in IgM secretion that follows it can be blocked by a dominant negative form of Blimp-1. At the same time, the ectopic expression of Blimp-1 in these partially activated cells induces an apoptotic response that also can be suppressed by the same dominant negative protein. A similar effect was noticed when Blimp-1 was expressed in the mature L10A and the immature WEHI-231 lines, indicating this may be a general effect at earlier stages of the B cell development, and distinct from the ability of Blimp-1 to induce maturation in late stages of differentiation. Truncation mutants indicate that the induction of the apoptotic response relies mainly on 69 amino acids within Blimp-1's proline-rich domain. We propose that Blimp-1 expression defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated at this point. |
format | Text |
id | pubmed-2212483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22124832008-04-16 Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development Messika, Eric J. Lu, Peter S. Sung, Yen-Jen Yao, Tony Chi, Jen-Tsan Chien, Yueh-hsiu Davis, Mark M. J Exp Med Articles The B lymphocyte–induced maturation protein (Blimp-1) upregulates the expression of syndecan-1 and J chain and represses that of c-myc. We have transfected Blimp-1 into two sublines of the BCL1 B cell lymphoma that represent distinct stages of B cell development in secondary lymphoid tissues. After interleukin (IL)-2 and IL-5 stimulation, the BCL1 3B3 cells differentiate into centrocyte-like cells, whereas the BCL1 5B1b cells blast and appear to be blocked at the centroblast stage. This blasting effect and the increase in IgM secretion that follows it can be blocked by a dominant negative form of Blimp-1. At the same time, the ectopic expression of Blimp-1 in these partially activated cells induces an apoptotic response that also can be suppressed by the same dominant negative protein. A similar effect was noticed when Blimp-1 was expressed in the mature L10A and the immature WEHI-231 lines, indicating this may be a general effect at earlier stages of the B cell development, and distinct from the ability of Blimp-1 to induce maturation in late stages of differentiation. Truncation mutants indicate that the induction of the apoptotic response relies mainly on 69 amino acids within Blimp-1's proline-rich domain. We propose that Blimp-1 expression defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated at this point. The Rockefeller University Press 1998-08-03 /pmc/articles/PMC2212483/ /pubmed/9687529 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Messika, Eric J. Lu, Peter S. Sung, Yen-Jen Yao, Tony Chi, Jen-Tsan Chien, Yueh-hsiu Davis, Mark M. Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title | Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title_full | Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title_fullStr | Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title_full_unstemmed | Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title_short | Differential Effect of B Lymphocyte–induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development |
title_sort | differential effect of b lymphocyte–induced maturation protein (blimp-1) expression on cell fate during b cell development |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212483/ https://www.ncbi.nlm.nih.gov/pubmed/9687529 |
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