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Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes

Dendritic cells, but not macrophages, efficiently phagocytose apoptotic cells and cross-present viral, tumor, and self-antigens to CD8(+) T cells. This in vitro pathway corresponds to the in vivo phenomena of cross-priming and cross-tolerance. Here, we demonstrate that phagocytosis of apoptotic cell...

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Autores principales: Albert, Matthew L., Pearce, S.Frieda A., Francisco, Loise M., Sauter, Birthe, Roy, Pampa, Silverstein, Roy L., Bhardwaj, Nina
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212488/
https://www.ncbi.nlm.nih.gov/pubmed/9763615
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author Albert, Matthew L.
Pearce, S.Frieda A.
Francisco, Loise M.
Sauter, Birthe
Roy, Pampa
Silverstein, Roy L.
Bhardwaj, Nina
author_facet Albert, Matthew L.
Pearce, S.Frieda A.
Francisco, Loise M.
Sauter, Birthe
Roy, Pampa
Silverstein, Roy L.
Bhardwaj, Nina
author_sort Albert, Matthew L.
collection PubMed
description Dendritic cells, but not macrophages, efficiently phagocytose apoptotic cells and cross-present viral, tumor, and self-antigens to CD8(+) T cells. This in vitro pathway corresponds to the in vivo phenomena of cross-priming and cross-tolerance. Here, we demonstrate that phagocytosis of apoptotic cells is restricted to the immature stage of dendritic cell (DC) development, and that this process is accompanied by the expression of a unique profile of receptors, in particular the α(v)β(5) integrin and CD36. Upon maturation, these receptors and, in turn, the phagocytic capacity of DCs, are downmodulated. Macrophages engulf apoptotic cells more efficiently than DCs, and although they express many receptors that mediate this uptake, they lack the α(v)β(5) integrin. Furthermore, in contrast to DCs, macrophages fail to cross-present antigenic material contained within the engulfed apoptotic cells. Thus, DCs use unique pathways for the phagocytosis, processing, and presentation of antigen derived from apoptotic cells on class I major histocompatibility complex. We suggest that the α(v)β(5) integrin plays a critical role in the trafficking of exogenous antigen by immature DCs in this cross-priming pathway.
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spelling pubmed-22124882008-04-16 Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes Albert, Matthew L. Pearce, S.Frieda A. Francisco, Loise M. Sauter, Birthe Roy, Pampa Silverstein, Roy L. Bhardwaj, Nina J Exp Med Articles Dendritic cells, but not macrophages, efficiently phagocytose apoptotic cells and cross-present viral, tumor, and self-antigens to CD8(+) T cells. This in vitro pathway corresponds to the in vivo phenomena of cross-priming and cross-tolerance. Here, we demonstrate that phagocytosis of apoptotic cells is restricted to the immature stage of dendritic cell (DC) development, and that this process is accompanied by the expression of a unique profile of receptors, in particular the α(v)β(5) integrin and CD36. Upon maturation, these receptors and, in turn, the phagocytic capacity of DCs, are downmodulated. Macrophages engulf apoptotic cells more efficiently than DCs, and although they express many receptors that mediate this uptake, they lack the α(v)β(5) integrin. Furthermore, in contrast to DCs, macrophages fail to cross-present antigenic material contained within the engulfed apoptotic cells. Thus, DCs use unique pathways for the phagocytosis, processing, and presentation of antigen derived from apoptotic cells on class I major histocompatibility complex. We suggest that the α(v)β(5) integrin plays a critical role in the trafficking of exogenous antigen by immature DCs in this cross-priming pathway. The Rockefeller University Press 1998-10-05 /pmc/articles/PMC2212488/ /pubmed/9763615 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Albert, Matthew L.
Pearce, S.Frieda A.
Francisco, Loise M.
Sauter, Birthe
Roy, Pampa
Silverstein, Roy L.
Bhardwaj, Nina
Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title_full Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title_fullStr Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title_full_unstemmed Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title_short Immature Dendritic Cells Phagocytose Apoptotic Cells via α(v)β(5) and CD36, and Cross-present Antigens to Cytotoxic T Lymphocytes
title_sort immature dendritic cells phagocytose apoptotic cells via α(v)β(5) and cd36, and cross-present antigens to cytotoxic t lymphocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212488/
https://www.ncbi.nlm.nih.gov/pubmed/9763615
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