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Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq
BACKGROUND: The male-specific region of the mouse Y chromosome long arm (MSYq) contains three known highly multi-copy X-Y homologous gene families, Ssty1/2, Sly and Asty. Deletions on MSYq lead to teratozoospermia and subfertility or infertility, with a sex ratio skew in the offspring of subfertile...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212661/ https://www.ncbi.nlm.nih.gov/pubmed/17892569 http://dx.doi.org/10.1186/1471-2148-7-171 |
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author | Ellis, Peter JI Ferguson, Lydia Clemente, Emily J Affara, Nabeel A |
author_facet | Ellis, Peter JI Ferguson, Lydia Clemente, Emily J Affara, Nabeel A |
author_sort | Ellis, Peter JI |
collection | PubMed |
description | BACKGROUND: The male-specific region of the mouse Y chromosome long arm (MSYq) contains three known highly multi-copy X-Y homologous gene families, Ssty1/2, Sly and Asty. Deletions on MSYq lead to teratozoospermia and subfertility or infertility, with a sex ratio skew in the offspring of subfertile MSYqdel males RESULTS: We report the highly unusual genomic structure of a novel MSYq locus, Orly, and a diverse set of spermatid-specific transcripts arising from copies of this locus. Orly is composed of partial copies of Ssty1, Asty and Sly arranged in sequence. The Ssty1- and Sly-derived segments are in antisense orientation relative to each other, leading to bi-directional transcription of Orly. Genome search and phylogenetic tree analysis is used to determine the order of events in mouse Yq evolution. We find that Orly is the most recent gene to arise on Yq, and that subsequently there was massive expansion in copy number of all Yq-linked genes. CONCLUSION: Orly has an unprecedented chimeric structure, and generates both "forward" (Orly) and "reverse" (Orlyos) transcripts arising from the promoters at each end of the locus. The region of overlap of known Orly and Orlyos transcripts is homologous to Sly intron 2. We propose that Orly may be involved in an intragenomic conflict between mouse X and Y chromosomes, and that this process underlies the massive expansion in copy number of the genes on MSYq and their X homologues. |
format | Text |
id | pubmed-2212661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22126612008-01-24 Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq Ellis, Peter JI Ferguson, Lydia Clemente, Emily J Affara, Nabeel A BMC Evol Biol Research Article BACKGROUND: The male-specific region of the mouse Y chromosome long arm (MSYq) contains three known highly multi-copy X-Y homologous gene families, Ssty1/2, Sly and Asty. Deletions on MSYq lead to teratozoospermia and subfertility or infertility, with a sex ratio skew in the offspring of subfertile MSYqdel males RESULTS: We report the highly unusual genomic structure of a novel MSYq locus, Orly, and a diverse set of spermatid-specific transcripts arising from copies of this locus. Orly is composed of partial copies of Ssty1, Asty and Sly arranged in sequence. The Ssty1- and Sly-derived segments are in antisense orientation relative to each other, leading to bi-directional transcription of Orly. Genome search and phylogenetic tree analysis is used to determine the order of events in mouse Yq evolution. We find that Orly is the most recent gene to arise on Yq, and that subsequently there was massive expansion in copy number of all Yq-linked genes. CONCLUSION: Orly has an unprecedented chimeric structure, and generates both "forward" (Orly) and "reverse" (Orlyos) transcripts arising from the promoters at each end of the locus. The region of overlap of known Orly and Orlyos transcripts is homologous to Sly intron 2. We propose that Orly may be involved in an intragenomic conflict between mouse X and Y chromosomes, and that this process underlies the massive expansion in copy number of the genes on MSYq and their X homologues. BioMed Central 2007-09-24 /pmc/articles/PMC2212661/ /pubmed/17892569 http://dx.doi.org/10.1186/1471-2148-7-171 Text en Copyright © 2007 Ellis et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ellis, Peter JI Ferguson, Lydia Clemente, Emily J Affara, Nabeel A Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title | Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title_full | Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title_fullStr | Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title_full_unstemmed | Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title_short | Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq |
title_sort | bidirectional transcription of a novel chimeric gene mapping to mouse chromosome yq |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212661/ https://www.ncbi.nlm.nih.gov/pubmed/17892569 http://dx.doi.org/10.1186/1471-2148-7-171 |
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