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Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages

Macrophages play a critical role in both innate and acquired immunity because of their unique ability to internalize, kill, and degrade bacterial pathogens through the process of phagocytosis. The adaptor protein, amphiphysin IIm, participates in phagocytosis and is transiently associated with early...

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Autores principales: Gold, Elizabeth S., Simmons, Randi M., Petersen, Timothy W., Campbell, Lee Ann, Kuo, Cho-Chou, Aderem, Alan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212749/
https://www.ncbi.nlm.nih.gov/pubmed/15337791
http://dx.doi.org/10.1084/jem.20040546
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author Gold, Elizabeth S.
Simmons, Randi M.
Petersen, Timothy W.
Campbell, Lee Ann
Kuo, Cho-Chou
Aderem, Alan
author_facet Gold, Elizabeth S.
Simmons, Randi M.
Petersen, Timothy W.
Campbell, Lee Ann
Kuo, Cho-Chou
Aderem, Alan
author_sort Gold, Elizabeth S.
collection PubMed
description Macrophages play a critical role in both innate and acquired immunity because of their unique ability to internalize, kill, and degrade bacterial pathogens through the process of phagocytosis. The adaptor protein, amphiphysin IIm, participates in phagocytosis and is transiently associated with early phagosomes. Certain pathogens, including Chlamydia pneumoniae, have evolved mechanisms to subvert macrophage phagosome maturation and, thus, are able to survive within these cells. We report here that, although amphiphysin IIm is usually only transiently associated with the phagosome, it is indefinitely retained on vacuoles containing C. pneumoniae. Under these wild-type conditions, C. pneumoniae do not elicit significant nitric oxide (NO) production and are not killed. Abrogation of amphiphysin IIm function results in C. pneumoniae–induced NO production and in the sterilization of the vacuole. The data suggest that C. pneumoniae retains amphiphysin IIm on the vacuole to survive within the macrophage.
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spelling pubmed-22127492008-03-11 Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages Gold, Elizabeth S. Simmons, Randi M. Petersen, Timothy W. Campbell, Lee Ann Kuo, Cho-Chou Aderem, Alan J Exp Med Article Macrophages play a critical role in both innate and acquired immunity because of their unique ability to internalize, kill, and degrade bacterial pathogens through the process of phagocytosis. The adaptor protein, amphiphysin IIm, participates in phagocytosis and is transiently associated with early phagosomes. Certain pathogens, including Chlamydia pneumoniae, have evolved mechanisms to subvert macrophage phagosome maturation and, thus, are able to survive within these cells. We report here that, although amphiphysin IIm is usually only transiently associated with the phagosome, it is indefinitely retained on vacuoles containing C. pneumoniae. Under these wild-type conditions, C. pneumoniae do not elicit significant nitric oxide (NO) production and are not killed. Abrogation of amphiphysin IIm function results in C. pneumoniae–induced NO production and in the sterilization of the vacuole. The data suggest that C. pneumoniae retains amphiphysin IIm on the vacuole to survive within the macrophage. The Rockefeller University Press 2004-09-06 /pmc/articles/PMC2212749/ /pubmed/15337791 http://dx.doi.org/10.1084/jem.20040546 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gold, Elizabeth S.
Simmons, Randi M.
Petersen, Timothy W.
Campbell, Lee Ann
Kuo, Cho-Chou
Aderem, Alan
Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title_full Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title_fullStr Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title_full_unstemmed Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title_short Amphiphysin IIm Is Required for Survival of Chlamydia pneumoniae in Macrophages
title_sort amphiphysin iim is required for survival of chlamydia pneumoniae in macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212749/
https://www.ncbi.nlm.nih.gov/pubmed/15337791
http://dx.doi.org/10.1084/jem.20040546
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