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NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145

The NK cell–activating receptor NKG2D interacts with three different cellular ligands, all of which are regulated by mouse cytomegalovirus (MCMV). We set out to define the viral gene product regulating murine UL16-binding protein-like transcript (MULT)-1, a newly described NKG2D ligand. We show that...

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Autores principales: Krmpotic, Astrid, Hasan, Milena, Loewendorf, Andrea, Saulig, Tanja, Halenius, Anne, Lenac, Tihana, Polic, Bojan, Bubic, Ivan, Kriegeskorte, Anja, Pernjak-Pugel, Ester, Messerle, Martin, Hengel, Hartmut, Busch, Dirk H., Koszinowski, Ulrich H., Jonjic, Stipan
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212792/
https://www.ncbi.nlm.nih.gov/pubmed/15642742
http://dx.doi.org/10.1084/jem.20041617
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author Krmpotic, Astrid
Hasan, Milena
Loewendorf, Andrea
Saulig, Tanja
Halenius, Anne
Lenac, Tihana
Polic, Bojan
Bubic, Ivan
Kriegeskorte, Anja
Pernjak-Pugel, Ester
Messerle, Martin
Hengel, Hartmut
Busch, Dirk H.
Koszinowski, Ulrich H.
Jonjic, Stipan
author_facet Krmpotic, Astrid
Hasan, Milena
Loewendorf, Andrea
Saulig, Tanja
Halenius, Anne
Lenac, Tihana
Polic, Bojan
Bubic, Ivan
Kriegeskorte, Anja
Pernjak-Pugel, Ester
Messerle, Martin
Hengel, Hartmut
Busch, Dirk H.
Koszinowski, Ulrich H.
Jonjic, Stipan
author_sort Krmpotic, Astrid
collection PubMed
description The NK cell–activating receptor NKG2D interacts with three different cellular ligands, all of which are regulated by mouse cytomegalovirus (MCMV). We set out to define the viral gene product regulating murine UL16-binding protein-like transcript (MULT)-1, a newly described NKG2D ligand. We show that MCMV infection strongly induces MULT-1 gene expression, but surface expression of this glycoprotein is nevertheless completely abolished by the virus. Screening a panel of MCMV deletion mutants defined the gene m145 as the viral regulator of MULT-1. The MCMV m145-encoded glycoprotein turned out to be necessary and sufficient to regulate MULT-1 by preventing plasma membrane residence of MULT-1. The importance of MULT-1 in NK cell regulation in vivo was confirmed by the attenuating effect of the m145 deletion that was lifted after NK cell depletion. Our findings underline the significance of escaping MULT-1/NKG2D signaling for viral survival and maintenance.
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spelling pubmed-22127922008-03-11 NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145 Krmpotic, Astrid Hasan, Milena Loewendorf, Andrea Saulig, Tanja Halenius, Anne Lenac, Tihana Polic, Bojan Bubic, Ivan Kriegeskorte, Anja Pernjak-Pugel, Ester Messerle, Martin Hengel, Hartmut Busch, Dirk H. Koszinowski, Ulrich H. Jonjic, Stipan J Exp Med Article The NK cell–activating receptor NKG2D interacts with three different cellular ligands, all of which are regulated by mouse cytomegalovirus (MCMV). We set out to define the viral gene product regulating murine UL16-binding protein-like transcript (MULT)-1, a newly described NKG2D ligand. We show that MCMV infection strongly induces MULT-1 gene expression, but surface expression of this glycoprotein is nevertheless completely abolished by the virus. Screening a panel of MCMV deletion mutants defined the gene m145 as the viral regulator of MULT-1. The MCMV m145-encoded glycoprotein turned out to be necessary and sufficient to regulate MULT-1 by preventing plasma membrane residence of MULT-1. The importance of MULT-1 in NK cell regulation in vivo was confirmed by the attenuating effect of the m145 deletion that was lifted after NK cell depletion. Our findings underline the significance of escaping MULT-1/NKG2D signaling for viral survival and maintenance. The Rockefeller University Press 2005-01-17 /pmc/articles/PMC2212792/ /pubmed/15642742 http://dx.doi.org/10.1084/jem.20041617 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Krmpotic, Astrid
Hasan, Milena
Loewendorf, Andrea
Saulig, Tanja
Halenius, Anne
Lenac, Tihana
Polic, Bojan
Bubic, Ivan
Kriegeskorte, Anja
Pernjak-Pugel, Ester
Messerle, Martin
Hengel, Hartmut
Busch, Dirk H.
Koszinowski, Ulrich H.
Jonjic, Stipan
NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title_full NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title_fullStr NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title_full_unstemmed NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title_short NK cell activation through the NKG2D ligand MULT-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
title_sort nk cell activation through the nkg2d ligand mult-1 is selectively prevented by the glycoprotein encoded by mouse cytomegalovirus gene m145
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212792/
https://www.ncbi.nlm.nih.gov/pubmed/15642742
http://dx.doi.org/10.1084/jem.20041617
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