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AID from bony fish catalyzes class switch recombination

Class switch recombination was the last of the lymphocyte-specific DNA modification reactions to appear in the evolution of the adaptive immune system. It is absent in cartilaginous and bony fish, and it is common to all tetrapods. Class switching is initiated by activation-induced cytidine deaminas...

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Autores principales: Barreto, Vasco M., Pan-Hammarstrom, Qiang, Zhao, Yaofeng, Hammarstrom, Lennart, Misulovin, Ziva, Nussenzweig, Michel C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212934/
https://www.ncbi.nlm.nih.gov/pubmed/16157688
http://dx.doi.org/10.1084/jem.20051378
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author Barreto, Vasco M.
Pan-Hammarstrom, Qiang
Zhao, Yaofeng
Hammarstrom, Lennart
Misulovin, Ziva
Nussenzweig, Michel C.
author_facet Barreto, Vasco M.
Pan-Hammarstrom, Qiang
Zhao, Yaofeng
Hammarstrom, Lennart
Misulovin, Ziva
Nussenzweig, Michel C.
author_sort Barreto, Vasco M.
collection PubMed
description Class switch recombination was the last of the lymphocyte-specific DNA modification reactions to appear in the evolution of the adaptive immune system. It is absent in cartilaginous and bony fish, and it is common to all tetrapods. Class switching is initiated by activation-induced cytidine deaminase (AID), an enzyme expressed in cartilaginous and bony fish that is also required for somatic hypermutation. Fish AID differs from orthologs found in tetrapods in several respects, including its catalytic domain and carboxy-terminal region, both of which are essential for the switching reaction. To determine whether evolution of class switch recombination required alterations in AID, we assayed AID from Japanese puffer and zebra fish for class-switching activity in mouse B cells. We find that fish AID catalyzes class switch recombination in mammalian B cells. Thus, AID had the potential to catalyze this reaction before the teleost and tetrapod lineages diverged, suggesting that the later appearance of a class-switching reaction was dependent on the evolution of switch regions and multiple constant regions in the IgH locus.
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spelling pubmed-22129342008-03-11 AID from bony fish catalyzes class switch recombination Barreto, Vasco M. Pan-Hammarstrom, Qiang Zhao, Yaofeng Hammarstrom, Lennart Misulovin, Ziva Nussenzweig, Michel C. J Exp Med Brief Definitive Report Class switch recombination was the last of the lymphocyte-specific DNA modification reactions to appear in the evolution of the adaptive immune system. It is absent in cartilaginous and bony fish, and it is common to all tetrapods. Class switching is initiated by activation-induced cytidine deaminase (AID), an enzyme expressed in cartilaginous and bony fish that is also required for somatic hypermutation. Fish AID differs from orthologs found in tetrapods in several respects, including its catalytic domain and carboxy-terminal region, both of which are essential for the switching reaction. To determine whether evolution of class switch recombination required alterations in AID, we assayed AID from Japanese puffer and zebra fish for class-switching activity in mouse B cells. We find that fish AID catalyzes class switch recombination in mammalian B cells. Thus, AID had the potential to catalyze this reaction before the teleost and tetrapod lineages diverged, suggesting that the later appearance of a class-switching reaction was dependent on the evolution of switch regions and multiple constant regions in the IgH locus. The Rockefeller University Press 2005-09-19 /pmc/articles/PMC2212934/ /pubmed/16157688 http://dx.doi.org/10.1084/jem.20051378 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Barreto, Vasco M.
Pan-Hammarstrom, Qiang
Zhao, Yaofeng
Hammarstrom, Lennart
Misulovin, Ziva
Nussenzweig, Michel C.
AID from bony fish catalyzes class switch recombination
title AID from bony fish catalyzes class switch recombination
title_full AID from bony fish catalyzes class switch recombination
title_fullStr AID from bony fish catalyzes class switch recombination
title_full_unstemmed AID from bony fish catalyzes class switch recombination
title_short AID from bony fish catalyzes class switch recombination
title_sort aid from bony fish catalyzes class switch recombination
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212934/
https://www.ncbi.nlm.nih.gov/pubmed/16157688
http://dx.doi.org/10.1084/jem.20051378
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