Cargando…
Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved i...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213058/ https://www.ncbi.nlm.nih.gov/pubmed/15728240 http://dx.doi.org/10.1084/jem.20041065 |
_version_ | 1782148815996846080 |
---|---|
author | Traidl-Hoffmann, Claudia Mariani, Valentina Hochrein, Hubertus Karg, Kathrin Wagner, Hermann Ring, Johannes Mueller, Martin J. Jakob, Thilo Behrendt, Heidrun |
author_facet | Traidl-Hoffmann, Claudia Mariani, Valentina Hochrein, Hubertus Karg, Kathrin Wagner, Hermann Ring, Johannes Mueller, Martin J. Jakob, Thilo Behrendt, Heidrun |
author_sort | Traidl-Hoffmann, Claudia |
collection | PubMed |
description | Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extracts (Bet.-APE) selectively inhibited interleukin (IL)-12 p70 production of lipopolysaccharide (LPS)- or CD40L-activated DC, whereas IL-6, IL-10, and TNFα remained unchanged. Presence of Bet.-APE during DC activation resulted in DC with increased T helper type 2 (Th2) cell and reduced Th1 cell polarizing capacity. Chemical analysis of Bet.-APE revealed the presence of phytoprostanes (dinor isoprostanes) with prostaglandin E(1)-, F(1)-, A(1)-, or B(1)-ring systems of which only E(1)-phytoprostanes dose dependently inhibited the LPS-induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC. These results suggest that pollen-derived E(1)-phytoprostanes not only resemble endogenous prostaglandin E(2) structurally but also functionally in that they act as regulators that modulate human DC function in a fashion that favors Th2 cell polarization. |
format | Text |
id | pubmed-2213058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22130582008-03-11 Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization Traidl-Hoffmann, Claudia Mariani, Valentina Hochrein, Hubertus Karg, Kathrin Wagner, Hermann Ring, Johannes Mueller, Martin J. Jakob, Thilo Behrendt, Heidrun J Exp Med Article Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extracts (Bet.-APE) selectively inhibited interleukin (IL)-12 p70 production of lipopolysaccharide (LPS)- or CD40L-activated DC, whereas IL-6, IL-10, and TNFα remained unchanged. Presence of Bet.-APE during DC activation resulted in DC with increased T helper type 2 (Th2) cell and reduced Th1 cell polarizing capacity. Chemical analysis of Bet.-APE revealed the presence of phytoprostanes (dinor isoprostanes) with prostaglandin E(1)-, F(1)-, A(1)-, or B(1)-ring systems of which only E(1)-phytoprostanes dose dependently inhibited the LPS-induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC. These results suggest that pollen-derived E(1)-phytoprostanes not only resemble endogenous prostaglandin E(2) structurally but also functionally in that they act as regulators that modulate human DC function in a fashion that favors Th2 cell polarization. The Rockefeller University Press 2005-02-21 /pmc/articles/PMC2213058/ /pubmed/15728240 http://dx.doi.org/10.1084/jem.20041065 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Traidl-Hoffmann, Claudia Mariani, Valentina Hochrein, Hubertus Karg, Kathrin Wagner, Hermann Ring, Johannes Mueller, Martin J. Jakob, Thilo Behrendt, Heidrun Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title | Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title_full | Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title_fullStr | Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title_full_unstemmed | Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title_short | Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization |
title_sort | pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment t helper type 2 cell polarization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213058/ https://www.ncbi.nlm.nih.gov/pubmed/15728240 http://dx.doi.org/10.1084/jem.20041065 |
work_keys_str_mv | AT traidlhoffmannclaudia pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT marianivalentina pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT hochreinhubertus pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT kargkathrin pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT wagnerhermann pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT ringjohannes pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT muellermartinj pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT jakobthilo pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization AT behrendtheidrun pollenassociatedphytoprostanesinhibitdendriticcellinterleukin12productionandaugmentthelpertype2cellpolarization |