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Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization

Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved i...

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Autores principales: Traidl-Hoffmann, Claudia, Mariani, Valentina, Hochrein, Hubertus, Karg, Kathrin, Wagner, Hermann, Ring, Johannes, Mueller, Martin J., Jakob, Thilo, Behrendt, Heidrun
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213058/
https://www.ncbi.nlm.nih.gov/pubmed/15728240
http://dx.doi.org/10.1084/jem.20041065
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author Traidl-Hoffmann, Claudia
Mariani, Valentina
Hochrein, Hubertus
Karg, Kathrin
Wagner, Hermann
Ring, Johannes
Mueller, Martin J.
Jakob, Thilo
Behrendt, Heidrun
author_facet Traidl-Hoffmann, Claudia
Mariani, Valentina
Hochrein, Hubertus
Karg, Kathrin
Wagner, Hermann
Ring, Johannes
Mueller, Martin J.
Jakob, Thilo
Behrendt, Heidrun
author_sort Traidl-Hoffmann, Claudia
collection PubMed
description Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extracts (Bet.-APE) selectively inhibited interleukin (IL)-12 p70 production of lipopolysaccharide (LPS)- or CD40L-activated DC, whereas IL-6, IL-10, and TNFα remained unchanged. Presence of Bet.-APE during DC activation resulted in DC with increased T helper type 2 (Th2) cell and reduced Th1 cell polarizing capacity. Chemical analysis of Bet.-APE revealed the presence of phytoprostanes (dinor isoprostanes) with prostaglandin E(1)-, F(1)-, A(1)-, or B(1)-ring systems of which only E(1)-phytoprostanes dose dependently inhibited the LPS-induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC. These results suggest that pollen-derived E(1)-phytoprostanes not only resemble endogenous prostaglandin E(2) structurally but also functionally in that they act as regulators that modulate human DC function in a fashion that favors Th2 cell polarization.
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spelling pubmed-22130582008-03-11 Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization Traidl-Hoffmann, Claudia Mariani, Valentina Hochrein, Hubertus Karg, Kathrin Wagner, Hermann Ring, Johannes Mueller, Martin J. Jakob, Thilo Behrendt, Heidrun J Exp Med Article Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extracts (Bet.-APE) selectively inhibited interleukin (IL)-12 p70 production of lipopolysaccharide (LPS)- or CD40L-activated DC, whereas IL-6, IL-10, and TNFα remained unchanged. Presence of Bet.-APE during DC activation resulted in DC with increased T helper type 2 (Th2) cell and reduced Th1 cell polarizing capacity. Chemical analysis of Bet.-APE revealed the presence of phytoprostanes (dinor isoprostanes) with prostaglandin E(1)-, F(1)-, A(1)-, or B(1)-ring systems of which only E(1)-phytoprostanes dose dependently inhibited the LPS-induced IL-12 p70 release and augmented the Th2 cell polarizing capacity of DC. These results suggest that pollen-derived E(1)-phytoprostanes not only resemble endogenous prostaglandin E(2) structurally but also functionally in that they act as regulators that modulate human DC function in a fashion that favors Th2 cell polarization. The Rockefeller University Press 2005-02-21 /pmc/articles/PMC2213058/ /pubmed/15728240 http://dx.doi.org/10.1084/jem.20041065 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Traidl-Hoffmann, Claudia
Mariani, Valentina
Hochrein, Hubertus
Karg, Kathrin
Wagner, Hermann
Ring, Johannes
Mueller, Martin J.
Jakob, Thilo
Behrendt, Heidrun
Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title_full Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title_fullStr Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title_full_unstemmed Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title_short Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization
title_sort pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment t helper type 2 cell polarization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213058/
https://www.ncbi.nlm.nih.gov/pubmed/15728240
http://dx.doi.org/10.1084/jem.20041065
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