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Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis

Circulating murine monocytes comprise two largely exclusive subpopulations that are responsible for seeding normal tissues (Gr-1(−)/CCR2(−)/CX(3)CR1(high)) or responding to sites of inflammation (Gr-1(+)/CCR2(+)/CX(3)CR1(lo)). Gr-1(+) monocytes are recruited to the site of infection during the early...

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Autores principales: Robben, Paul M., LaRegina, Marie, Kuziel, William A., Sibley, L. David
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213275/
https://www.ncbi.nlm.nih.gov/pubmed/15928200
http://dx.doi.org/10.1084/jem.20050054
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author Robben, Paul M.
LaRegina, Marie
Kuziel, William A.
Sibley, L. David
author_facet Robben, Paul M.
LaRegina, Marie
Kuziel, William A.
Sibley, L. David
author_sort Robben, Paul M.
collection PubMed
description Circulating murine monocytes comprise two largely exclusive subpopulations that are responsible for seeding normal tissues (Gr-1(−)/CCR2(−)/CX(3)CR1(high)) or responding to sites of inflammation (Gr-1(+)/CCR2(+)/CX(3)CR1(lo)). Gr-1(+) monocytes are recruited to the site of infection during the early stages of immune response to the intracellular pathogen Toxoplasma gondii. A murine model of toxoplasmosis was thus used to examine the importance of Gr-1(+) monocytes in the control of disseminated parasitic infection in vivo. The recruitment of Gr-1(+) monocytes was intimately associated with the ability to suppress early parasite replication at the site of inoculation. Infection of CCR2(−/−) and MCP-1(−/−) mice with typically nonlethal, low doses of T. gondii resulted in the abrogated recruitment of Gr-1(+) monocytes. The failure to recruit Gr-1(+) monocytes resulted in greatly enhanced mortality despite the induction of normal Th1 cell responses leading to high levels of IL-12, TNF-α, and IFN-γ. The profound susceptibility of CCR2(−/−) mice establishes Gr-1(+) monocytes as necessary effector cells in the resistance to acute toxoplasmosis and suggests that the CCR2-dependent recruitment of Gr-1(+) monocytes may be an important general mechanism for resistance to intracellular pathogens.
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spelling pubmed-22132752008-03-11 Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis Robben, Paul M. LaRegina, Marie Kuziel, William A. Sibley, L. David J Exp Med Article Circulating murine monocytes comprise two largely exclusive subpopulations that are responsible for seeding normal tissues (Gr-1(−)/CCR2(−)/CX(3)CR1(high)) or responding to sites of inflammation (Gr-1(+)/CCR2(+)/CX(3)CR1(lo)). Gr-1(+) monocytes are recruited to the site of infection during the early stages of immune response to the intracellular pathogen Toxoplasma gondii. A murine model of toxoplasmosis was thus used to examine the importance of Gr-1(+) monocytes in the control of disseminated parasitic infection in vivo. The recruitment of Gr-1(+) monocytes was intimately associated with the ability to suppress early parasite replication at the site of inoculation. Infection of CCR2(−/−) and MCP-1(−/−) mice with typically nonlethal, low doses of T. gondii resulted in the abrogated recruitment of Gr-1(+) monocytes. The failure to recruit Gr-1(+) monocytes resulted in greatly enhanced mortality despite the induction of normal Th1 cell responses leading to high levels of IL-12, TNF-α, and IFN-γ. The profound susceptibility of CCR2(−/−) mice establishes Gr-1(+) monocytes as necessary effector cells in the resistance to acute toxoplasmosis and suggests that the CCR2-dependent recruitment of Gr-1(+) monocytes may be an important general mechanism for resistance to intracellular pathogens. The Rockefeller University Press 2005-06-06 /pmc/articles/PMC2213275/ /pubmed/15928200 http://dx.doi.org/10.1084/jem.20050054 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Robben, Paul M.
LaRegina, Marie
Kuziel, William A.
Sibley, L. David
Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title_full Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title_fullStr Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title_full_unstemmed Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title_short Recruitment of Gr-1(+) monocytes is essential for control of acute toxoplasmosis
title_sort recruitment of gr-1(+) monocytes is essential for control of acute toxoplasmosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213275/
https://www.ncbi.nlm.nih.gov/pubmed/15928200
http://dx.doi.org/10.1084/jem.20050054
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