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Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
T cell hybridomas isolated from nonresponder H-2(b) mice immunized with pork insulin were stimulated by insulin in the presence of major histocompatibility complex (MHC)-unmatched antigen presenting cells. The restriction element used by these CD4(−) T cells was mapped to an oligomorphic MHC class I...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213382/ https://www.ncbi.nlm.nih.gov/pubmed/9730897 |
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author | Mark Tompkins, S. Kraft, Jennifer R. Dao, Chinh T. Soloski, Mark J. Jensen, Peter E. |
author_facet | Mark Tompkins, S. Kraft, Jennifer R. Dao, Chinh T. Soloski, Mark J. Jensen, Peter E. |
author_sort | Mark Tompkins, S. |
collection | PubMed |
description | T cell hybridomas isolated from nonresponder H-2(b) mice immunized with pork insulin were stimulated by insulin in the presence of major histocompatibility complex (MHC)-unmatched antigen presenting cells. The restriction element used by these CD4(−) T cells was mapped to an oligomorphic MHC class Ib protein encoded in the T region and identified as Qa-1(b) using transfectants. The antigenic determinant was localized to the insulin B chain, and experiments with truncated peptides suggested that it is unexpectedly long, comprising most or all of the 30 amino acid B chain. The antigen processing pathway used to present insulin to the Qa-1(b)– restricted T cells does not require transporters associated with antigen processing (TAP), and it is inhibited by chloroquine. A wide variety of cell lines from different tissues efficiently present soluble insulin to Qa-1(b)–restricted T cells, and insulin presentation is not enhanced by phagocytic stimuli. Our results demonstrate that Qa-1(b) can function to present exogenous protein to T cells in a manner similar to MHC class II molecules. Therefore, this class Ib protein may have access to a novel antigen processing pathway that is not available to class Ia molecules. |
format | Text |
id | pubmed-2213382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22133822008-04-16 Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b) Mark Tompkins, S. Kraft, Jennifer R. Dao, Chinh T. Soloski, Mark J. Jensen, Peter E. J Exp Med Articles T cell hybridomas isolated from nonresponder H-2(b) mice immunized with pork insulin were stimulated by insulin in the presence of major histocompatibility complex (MHC)-unmatched antigen presenting cells. The restriction element used by these CD4(−) T cells was mapped to an oligomorphic MHC class Ib protein encoded in the T region and identified as Qa-1(b) using transfectants. The antigenic determinant was localized to the insulin B chain, and experiments with truncated peptides suggested that it is unexpectedly long, comprising most or all of the 30 amino acid B chain. The antigen processing pathway used to present insulin to the Qa-1(b)– restricted T cells does not require transporters associated with antigen processing (TAP), and it is inhibited by chloroquine. A wide variety of cell lines from different tissues efficiently present soluble insulin to Qa-1(b)–restricted T cells, and insulin presentation is not enhanced by phagocytic stimuli. Our results demonstrate that Qa-1(b) can function to present exogenous protein to T cells in a manner similar to MHC class II molecules. Therefore, this class Ib protein may have access to a novel antigen processing pathway that is not available to class Ia molecules. The Rockefeller University Press 1998-09-07 /pmc/articles/PMC2213382/ /pubmed/9730897 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Mark Tompkins, S. Kraft, Jennifer R. Dao, Chinh T. Soloski, Mark J. Jensen, Peter E. Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b) |
title | Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
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title_full | Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
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title_fullStr | Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
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title_full_unstemmed | Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
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title_short | Transporters Associated with Antigen Processing (TAP)-independent Presentation of Soluble Insulin to α/β T Cells by the Class Ib Gene Product, Qa-1(b)
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title_sort | transporters associated with antigen processing (tap)-independent presentation of soluble insulin to α/β t cells by the class ib gene product, qa-1(b) |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213382/ https://www.ncbi.nlm.nih.gov/pubmed/9730897 |
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