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Virus-Induced Maturation and Activation of Autoreactive Memory B Cells
We have examined B cell populations that participate in distinct phases of the immune response to the influenza virus A/PR/8/34 hemagglutinin (HA) for their susceptibility to negative selection in mice that express the HA as a neo–self-antigen (HA104 mice). We demonstrated previously that specificit...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213501/ https://www.ncbi.nlm.nih.gov/pubmed/11120773 |
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author | Reed, Amy J. Riley, Michael P. Caton, Andrew J. |
author_facet | Reed, Amy J. Riley, Michael P. Caton, Andrew J. |
author_sort | Reed, Amy J. |
collection | PubMed |
description | We have examined B cell populations that participate in distinct phases of the immune response to the influenza virus A/PR/8/34 hemagglutinin (HA) for their susceptibility to negative selection in mice that express the HA as a neo–self-antigen (HA104 mice). We demonstrated previously that specificity for the neo–self-HA causes a population of immunoglobulin G antibody-secreting cells, which dominate the primary response to virus immunization in BALB/c mice, to be negatively selected in HA104 mice. We find here that in contrast to these primary response B cells, HA-specific memory response B cells developed equivalently in HA104 and nontransgenic (BALB/c) mice. Indeed, there was no indication that HA-specific B cells were negatively selected during memory formation in influenza virus–immunized HA104 mice, even though the neo–self-HA can be recognized by memory B cells. Furthermore, HA-specific autoantibodies were induced in the absence of virus immunization by mating HA104 mice with mice transgenic for a CD4(+) HA-specific T cell receptor. These findings indicate that specificity for a self-antigen does not prevent the maturation of autoreactive B cells in the germinal center pathway. Rather, the availability of CD4(+) T cell help may play a crucial role in regulating autoantibody responses to the HA in HA104 mice. |
format | Text |
id | pubmed-2213501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22135012008-04-16 Virus-Induced Maturation and Activation of Autoreactive Memory B Cells Reed, Amy J. Riley, Michael P. Caton, Andrew J. J Exp Med Original Article We have examined B cell populations that participate in distinct phases of the immune response to the influenza virus A/PR/8/34 hemagglutinin (HA) for their susceptibility to negative selection in mice that express the HA as a neo–self-antigen (HA104 mice). We demonstrated previously that specificity for the neo–self-HA causes a population of immunoglobulin G antibody-secreting cells, which dominate the primary response to virus immunization in BALB/c mice, to be negatively selected in HA104 mice. We find here that in contrast to these primary response B cells, HA-specific memory response B cells developed equivalently in HA104 and nontransgenic (BALB/c) mice. Indeed, there was no indication that HA-specific B cells were negatively selected during memory formation in influenza virus–immunized HA104 mice, even though the neo–self-HA can be recognized by memory B cells. Furthermore, HA-specific autoantibodies were induced in the absence of virus immunization by mating HA104 mice with mice transgenic for a CD4(+) HA-specific T cell receptor. These findings indicate that specificity for a self-antigen does not prevent the maturation of autoreactive B cells in the germinal center pathway. Rather, the availability of CD4(+) T cell help may play a crucial role in regulating autoantibody responses to the HA in HA104 mice. The Rockefeller University Press 2000-12-18 /pmc/articles/PMC2213501/ /pubmed/11120773 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Reed, Amy J. Riley, Michael P. Caton, Andrew J. Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title | Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title_full | Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title_fullStr | Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title_full_unstemmed | Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title_short | Virus-Induced Maturation and Activation of Autoreactive Memory B Cells |
title_sort | virus-induced maturation and activation of autoreactive memory b cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213501/ https://www.ncbi.nlm.nih.gov/pubmed/11120773 |
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