Cargando…

Cohesin Smc1β determines meiotic chromatin axis loop organization

Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is...

Descripción completa

Detalles Bibliográficos
Autores principales: Novak, Ivana, Wang, Hong, Revenkova, Ekaterina, Jessberger, Rolf, Scherthan, Harry, Höög, Christer
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213612/
https://www.ncbi.nlm.nih.gov/pubmed/18180366
http://dx.doi.org/10.1083/jcb.200706136
_version_ 1782148918351495168
author Novak, Ivana
Wang, Hong
Revenkova, Ekaterina
Jessberger, Rolf
Scherthan, Harry
Höög, Christer
author_facet Novak, Ivana
Wang, Hong
Revenkova, Ekaterina
Jessberger, Rolf
Scherthan, Harry
Höög, Christer
author_sort Novak, Ivana
collection PubMed
description Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is largely unknown. We use two mouse model systems in which the postreplicative meiotic chromosome axes in the mutant oocytes are either longer or shorter than in wild-type oocytes. We observe a strict correlation between chromosome axis extension and a general and reciprocal shortening of chromatin loop size. However, in oocytes with a shorter chromosome axis, only a subset of the chromatin loops is extended. We find that the changes in chromatin loop size observed in oocytes with shorter or longer chromosome axes depend on the structural maintenance of chromosomes 1β (Smc1β), a mammalian chromosome–associated meiosis-specific cohesin. Our results suggest that in addition to its role in sister chromatid cohesion, Smc1β determines meiotic chromatin loop organization.
format Text
id pubmed-2213612
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22136122008-07-14 Cohesin Smc1β determines meiotic chromatin axis loop organization Novak, Ivana Wang, Hong Revenkova, Ekaterina Jessberger, Rolf Scherthan, Harry Höög, Christer J Cell Biol Research Articles Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is largely unknown. We use two mouse model systems in which the postreplicative meiotic chromosome axes in the mutant oocytes are either longer or shorter than in wild-type oocytes. We observe a strict correlation between chromosome axis extension and a general and reciprocal shortening of chromatin loop size. However, in oocytes with a shorter chromosome axis, only a subset of the chromatin loops is extended. We find that the changes in chromatin loop size observed in oocytes with shorter or longer chromosome axes depend on the structural maintenance of chromosomes 1β (Smc1β), a mammalian chromosome–associated meiosis-specific cohesin. Our results suggest that in addition to its role in sister chromatid cohesion, Smc1β determines meiotic chromatin loop organization. The Rockefeller University Press 2008-01-14 /pmc/articles/PMC2213612/ /pubmed/18180366 http://dx.doi.org/10.1083/jcb.200706136 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Novak, Ivana
Wang, Hong
Revenkova, Ekaterina
Jessberger, Rolf
Scherthan, Harry
Höög, Christer
Cohesin Smc1β determines meiotic chromatin axis loop organization
title Cohesin Smc1β determines meiotic chromatin axis loop organization
title_full Cohesin Smc1β determines meiotic chromatin axis loop organization
title_fullStr Cohesin Smc1β determines meiotic chromatin axis loop organization
title_full_unstemmed Cohesin Smc1β determines meiotic chromatin axis loop organization
title_short Cohesin Smc1β determines meiotic chromatin axis loop organization
title_sort cohesin smc1β determines meiotic chromatin axis loop organization
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213612/
https://www.ncbi.nlm.nih.gov/pubmed/18180366
http://dx.doi.org/10.1083/jcb.200706136
work_keys_str_mv AT novakivana cohesinsmc1bdeterminesmeioticchromatinaxislooporganization
AT wanghong cohesinsmc1bdeterminesmeioticchromatinaxislooporganization
AT revenkovaekaterina cohesinsmc1bdeterminesmeioticchromatinaxislooporganization
AT jessbergerrolf cohesinsmc1bdeterminesmeioticchromatinaxislooporganization
AT scherthanharry cohesinsmc1bdeterminesmeioticchromatinaxislooporganization
AT hoogchrister cohesinsmc1bdeterminesmeioticchromatinaxislooporganization