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Cohesin Smc1β determines meiotic chromatin axis loop organization
Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213612/ https://www.ncbi.nlm.nih.gov/pubmed/18180366 http://dx.doi.org/10.1083/jcb.200706136 |
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author | Novak, Ivana Wang, Hong Revenkova, Ekaterina Jessberger, Rolf Scherthan, Harry Höög, Christer |
author_facet | Novak, Ivana Wang, Hong Revenkova, Ekaterina Jessberger, Rolf Scherthan, Harry Höög, Christer |
author_sort | Novak, Ivana |
collection | PubMed |
description | Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is largely unknown. We use two mouse model systems in which the postreplicative meiotic chromosome axes in the mutant oocytes are either longer or shorter than in wild-type oocytes. We observe a strict correlation between chromosome axis extension and a general and reciprocal shortening of chromatin loop size. However, in oocytes with a shorter chromosome axis, only a subset of the chromatin loops is extended. We find that the changes in chromatin loop size observed in oocytes with shorter or longer chromosome axes depend on the structural maintenance of chromosomes 1β (Smc1β), a mammalian chromosome–associated meiosis-specific cohesin. Our results suggest that in addition to its role in sister chromatid cohesion, Smc1β determines meiotic chromatin loop organization. |
format | Text |
id | pubmed-2213612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22136122008-07-14 Cohesin Smc1β determines meiotic chromatin axis loop organization Novak, Ivana Wang, Hong Revenkova, Ekaterina Jessberger, Rolf Scherthan, Harry Höög, Christer J Cell Biol Research Articles Meiotic chromosomes consist of proteinaceous axial structures from which chromatin loops emerge. Although we know that loop density along the meiotic chromosome axis is conserved in organisms with different genome sizes, the basis for the regular spacing of chromatin loops and their organization is largely unknown. We use two mouse model systems in which the postreplicative meiotic chromosome axes in the mutant oocytes are either longer or shorter than in wild-type oocytes. We observe a strict correlation between chromosome axis extension and a general and reciprocal shortening of chromatin loop size. However, in oocytes with a shorter chromosome axis, only a subset of the chromatin loops is extended. We find that the changes in chromatin loop size observed in oocytes with shorter or longer chromosome axes depend on the structural maintenance of chromosomes 1β (Smc1β), a mammalian chromosome–associated meiosis-specific cohesin. Our results suggest that in addition to its role in sister chromatid cohesion, Smc1β determines meiotic chromatin loop organization. The Rockefeller University Press 2008-01-14 /pmc/articles/PMC2213612/ /pubmed/18180366 http://dx.doi.org/10.1083/jcb.200706136 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Novak, Ivana Wang, Hong Revenkova, Ekaterina Jessberger, Rolf Scherthan, Harry Höög, Christer Cohesin Smc1β determines meiotic chromatin axis loop organization |
title | Cohesin Smc1β determines meiotic chromatin axis loop organization |
title_full | Cohesin Smc1β determines meiotic chromatin axis loop organization |
title_fullStr | Cohesin Smc1β determines meiotic chromatin axis loop organization |
title_full_unstemmed | Cohesin Smc1β determines meiotic chromatin axis loop organization |
title_short | Cohesin Smc1β determines meiotic chromatin axis loop organization |
title_sort | cohesin smc1β determines meiotic chromatin axis loop organization |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213612/ https://www.ncbi.nlm.nih.gov/pubmed/18180366 http://dx.doi.org/10.1083/jcb.200706136 |
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