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Quantitation of conductance pathways in antral gastric mucosa
The magnitude of cellular and shunt conductance of Necturus gastric antral mucosa was studied by (a) comparing the cellular PD response to transepithelial PD response during changes of ionic activity in the serosal bathing solution and (b) by measurement of current spread within the epithelial sheet...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1975
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214887/ https://www.ncbi.nlm.nih.gov/pubmed/1176941 |
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collection | PubMed |
description | The magnitude of cellular and shunt conductance of Necturus gastric antral mucosa was studied by (a) comparing the cellular PD response to transepithelial PD response during changes of ionic activity in the serosal bathing solution and (b) by measurement of current spread within the epithelial sheet. Using constant product KCl changes cellular resistance was 6,788 omegacm2 and shunt resistance was 1,803 omegacm2. Deletion of HCO3- from the serosal solution produced similar but quantitatively smaller changes in PD. Using HCO3- deletion cellular resistance was 7,338 omegacm2 and shunt resistance was 1,973 omegacm2. Measurement of current spead within the mucosa avoids changing ionic gradients yet gave very similar results; cellular resistance was 8,967 omegacm2 and shunt resistance was 2,947 omegacm2. The shunt contribution to transepithelial conductance ranged from 75.2 to 79.0%. Shunt selectivity was assessed using KCl dilution potentials, where mucosal dilution gave a small change in tissue PD compatible with an anion/cation selectivity ratio of 1.16 across the shunt, whereas serosal dilution effect was dominated by a PD change across the serosal membrane of the cell. |
format | Text |
id | pubmed-2214887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1975 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22148872008-04-23 Quantitation of conductance pathways in antral gastric mucosa J Gen Physiol Articles The magnitude of cellular and shunt conductance of Necturus gastric antral mucosa was studied by (a) comparing the cellular PD response to transepithelial PD response during changes of ionic activity in the serosal bathing solution and (b) by measurement of current spread within the epithelial sheet. Using constant product KCl changes cellular resistance was 6,788 omegacm2 and shunt resistance was 1,803 omegacm2. Deletion of HCO3- from the serosal solution produced similar but quantitatively smaller changes in PD. Using HCO3- deletion cellular resistance was 7,338 omegacm2 and shunt resistance was 1,973 omegacm2. Measurement of current spead within the mucosa avoids changing ionic gradients yet gave very similar results; cellular resistance was 8,967 omegacm2 and shunt resistance was 2,947 omegacm2. The shunt contribution to transepithelial conductance ranged from 75.2 to 79.0%. Shunt selectivity was assessed using KCl dilution potentials, where mucosal dilution gave a small change in tissue PD compatible with an anion/cation selectivity ratio of 1.16 across the shunt, whereas serosal dilution effect was dominated by a PD change across the serosal membrane of the cell. The Rockefeller University Press 1975-05-01 /pmc/articles/PMC2214887/ /pubmed/1176941 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Quantitation of conductance pathways in antral gastric mucosa |
title | Quantitation of conductance pathways in antral gastric mucosa |
title_full | Quantitation of conductance pathways in antral gastric mucosa |
title_fullStr | Quantitation of conductance pathways in antral gastric mucosa |
title_full_unstemmed | Quantitation of conductance pathways in antral gastric mucosa |
title_short | Quantitation of conductance pathways in antral gastric mucosa |
title_sort | quantitation of conductance pathways in antral gastric mucosa |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214887/ https://www.ncbi.nlm.nih.gov/pubmed/1176941 |