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Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts
This paper is concerned with analyzing the sidedness of action of various determinants which alter the rate of ouabain binding to human red blood cell ghosts. Thus, ouabain binding promoted by orthophosphate (Pi) and its inhibition by Na are shown to be due to inside Pi and inside Na. External K inh...
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Lenguaje: | English |
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The Rockefeller University Press
1976
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214954/ https://www.ncbi.nlm.nih.gov/pubmed/1271041 |
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collection | PubMed |
description | This paper is concerned with analyzing the sidedness of action of various determinants which alter the rate of ouabain binding to human red blood cell ghosts. Thus, ouabain binding promoted by orthophosphate (Pi) and its inhibition by Na are shown to be due to inside Pi and inside Na. External K inhibits Pi-promoted ouabain binding and Nao acts to decrease the effectiveness of Ko. Similarly, inside uridine triphosphate (UTPi) stimulates the rate of ouabain binding which can be antagonized by either Nai or Ko acting alone. The actions of Nai and Ko are different when ouabain binding is promoted by Pi and UTPi compared to inside adenosine triphosphate (ATPi). With ATPi, the ouabain binding rate is only affected when Nai and Ko are both present. Possible differences in the mechanism of action of K and Na on Pi-and UTP- promoted binding are discussed in the light of their sidedness of action. |
format | Text |
id | pubmed-2214954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1976 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22149542008-04-23 Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts J Gen Physiol Articles This paper is concerned with analyzing the sidedness of action of various determinants which alter the rate of ouabain binding to human red blood cell ghosts. Thus, ouabain binding promoted by orthophosphate (Pi) and its inhibition by Na are shown to be due to inside Pi and inside Na. External K inhibits Pi-promoted ouabain binding and Nao acts to decrease the effectiveness of Ko. Similarly, inside uridine triphosphate (UTPi) stimulates the rate of ouabain binding which can be antagonized by either Nai or Ko acting alone. The actions of Nai and Ko are different when ouabain binding is promoted by Pi and UTPi compared to inside adenosine triphosphate (ATPi). With ATPi, the ouabain binding rate is only affected when Nai and Ko are both present. Possible differences in the mechanism of action of K and Na on Pi-and UTP- promoted binding are discussed in the light of their sidedness of action. The Rockefeller University Press 1976-05-01 /pmc/articles/PMC2214954/ /pubmed/1271041 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title | Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title_full | Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title_fullStr | Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title_full_unstemmed | Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title_short | Comparison of the side-dependent effects of Na and K on orthophosphate- , UTP-, and ATP-promoted ouabain binding to reconstituted human red blood cell ghosts |
title_sort | comparison of the side-dependent effects of na and k on orthophosphate- , utp-, and atp-promoted ouabain binding to reconstituted human red blood cell ghosts |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2214954/ https://www.ncbi.nlm.nih.gov/pubmed/1271041 |