Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis

Intracellular pH was recorded in immature starfish oocytes using pH- sensitive microelectrodes, and inwardly rectifying potassium currents were measured under voltage clamp. When the intracellular pH was lowered using acetate-buffered artificial sea water from the normal value of 7.09 to 5.9, inward...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2215492/
https://www.ncbi.nlm.nih.gov/pubmed/7061984
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description Intracellular pH was recorded in immature starfish oocytes using pH- sensitive microelectrodes, and inwardly rectifying potassium currents were measured under voltage clamp. When the intracellular pH was lowered using acetate-buffered artificial sea water from the normal value of 7.09 to 5.9, inward rectification was completely blocked. The relationship between inward rectification and internal pH between 7.09 and 5.9 could be fit by a titration curve for the binding of three H ions to a site with a pK of 6.26 to block the channel. The H+ block showed no voltage dependence, and the activation kinetics of the inwardly rectifying currents were not affected by the changes in internal pH.
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spelling pubmed-22154922008-04-23 Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis J Gen Physiol Articles Intracellular pH was recorded in immature starfish oocytes using pH- sensitive microelectrodes, and inwardly rectifying potassium currents were measured under voltage clamp. When the intracellular pH was lowered using acetate-buffered artificial sea water from the normal value of 7.09 to 5.9, inward rectification was completely blocked. The relationship between inward rectification and internal pH between 7.09 and 5.9 could be fit by a titration curve for the binding of three H ions to a site with a pK of 6.26 to block the channel. The H+ block showed no voltage dependence, and the activation kinetics of the inwardly rectifying currents were not affected by the changes in internal pH. The Rockefeller University Press 1982-01-01 /pmc/articles/PMC2215492/ /pubmed/7061984 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title_full Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title_fullStr Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title_full_unstemmed Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title_short Block of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
title_sort block of inward rectification by intracellular h+ in immature oocytes of the starfish mediaster aequalis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2215492/
https://www.ncbi.nlm.nih.gov/pubmed/7061984

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